Biosensor and method for manufacturing same

• US 7,727,367 B2
• Filed: 08/08/2003
• Issued: 06/01/2010
• Est. Priority Date: 08/13/2002
• Status: Expired due to Fees

• Current Assignee: Gunze Limited
• Original Assignee: Gunze Limited
• Inventors: Maruo, Masaharu, Deguchi, Tetsuji
• Primary Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US10/524,380
• Publication Number:
  US 20050265897A1
• Time in Patent Office: 2,489 Days
• Field of Search: 20440301-40315, 205/777.5, 205/778, 205/792
• US Class Current: 204/403.02
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence

Abstract

The present invention provides a biosensor that can be used for easy and quick quantitative analysis of a specific component in a sample with high precision and a method for manufacturing such a biosensor. The present invention also provides a biosensor that can be used for a highly precise quantitative analysis after an extended period of storage, specifically, a biosensor with excellent storage stability, and a method for manufacturing such a biosensor. The present invention relates to a biosensor comprising an electrically insulating substrate (1); an electrode (2) having a working electrode (21) and a counter electrode (22) formed on the substrate; and a reaction part (4) that is adhered to one end of the electrode (2); the reaction part (4) being mainly composed of a hydrophilic polymer comprising an oxidoreductase, an electron acceptor, fine crystalline cellulose powder, and hydrophilic and hydrophobic segments.

Claims

1. A biosensor comprising:

in its tip portion, an electrically insulating substrate and a cover sheet facing each other with a space in between and a spacer sheet somewhere therebetween; and

a reaction part having an oxidoreductase in a holding space formed by the substrate, the cover sheet and a spacer sheet end;

the liquid sample being delivered from the tip of the sensor into the holding space by capillary action, and an electrochemical change caused by an enzyme reaction between the liquid sample and the reaction part being detected using an electrode set having a working electrode and a counter electrode;

the biosensor being provided with a projection at only one side in the widthwise direction of the spacer sheet end in the holding space with the projection extending toward the tip portion of the biosensor;

the biosensor lacking an outlet for discharging gas in the holding space;

the spacer sheet consisting of one sheet; and

the electrode being disposed on the substrate.

2. A biosensor according to claim 1, wherein an inside corner part is formed on the spacer sheet end and is provided on the basal portion of the projection so as to enlarge the capacity of the holding space.

3. A method for measuring the glucose component, alcohol component, lactic acid component or uric acid component in a sample solution comprising:

exposing the sample solution to the biosensor of claim 1, and

measuring the quantity of glucose component, alcohol component, lactic acid component or uric acid component in said sample solution.

4. A biosensor according to claim 1, wherein the working electrode and the counter electrode are arranged approximately in parallel along the longitudinal direction of the substrate.

5. A biosensor according to claim 1, wherein the tip portion of the sensor is formed approximately in a semicircular shape.