Systems and methods for optical actuation of microfluidics based on OPTO-electrowetting

• US 7,727,771 B2
• Filed: 08/22/2005
• Issued: 06/01/2010
• Est. Priority Date: 05/31/2002
• Status: Active Grant

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Wu, Ming C., Chiou, Pei Yu
• Primary Examiner(s): Gordon; Brian R
• Application Number:
  US11/209,587
• Publication Number:
  US 20070243110A1
• Time in Patent Office: 1,744 Days
• Field of Search: 422 50-104, 204600-602, 204/603, 436/180, 436/164
• US Class Current: 436/164
• CPC Class Codes: B01L 2300/089 Virtual walls for guiding liquids
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 2400/0427 Electrowetting
• CPC Class Codes: B01L 3/502792 for moving individual droplets on a plate, e.g. by locally altering surface tension
• CPC Class Codes: F04B 19/006 Micropumps F04B43/043 and F04B43/095 take precedence
• CPC Class Codes: G01N 2035/1034 Transferring microquantities of liquid
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

The invention is related to methods and apparatus that manipulate droplets in a microfluidic environment. Advantageously, embodiments of the invention manipulate droplets by controlling the electro-wetting characteristics of a surface with light, thereby inducing a gradient in the surface tension of a droplet. The gradient in the surface tension propels the droplet by capillary force. A variety of operations, such as transporting, joining, cutting, and creating can be performed. Advantageously, embodiments of the invention obviate the need to create a relatively large and complex control electrode array. A plurality of photoconductive cells or a layer of a photoconductive material selectively couples an electrode carrying an electrical bias to otherwise floating conductive cells in response to a beam of light. The electrical bias applied to the conductive cell generates a localized electric field, which can change the contact angle of the droplet, thereby permitting the droplet to be propelled.

Claims

1. A method of manipulating a droplet, comprising:

providing a first plate and a second plate electrically coupled to a bias voltage;

said first and second plates having opposing surfaces;

placing at least one droplet between the opposing surfaces of the first and second plates; and

selectively illuminating portions of at least one opposing surface of the first plate or the second plate such that the illuminated portion induces a gradient in surface tension of the droplet;

wherein the gradient in the surface tension imparts motion to the droplet, thereby facilitating manipulation of the droplet.

2. A method as recited in claim 1, wherein said manipulation is selected from the group consisting essentially of dividing said droplet, joining said droplet with one or more other droplets, creating a droplet from a reservoir of liquid and moving said droplet from a first position to a second position.

3. A method as recited in claim 1, wherein said droplet is placed between opposing surfaces of said first and second plates, said surface of said first plate further comprising a hydrophobic surface.

4. A method as recited in claim 1:

wherein a cavity is formed with a plurality of spacers interposed between said first

and second plates; and

wherein said droplet is positioned in said cavity for manipulation.

5. A method as recited in claim 1, wherein at least one of said opposing surfaces comprises a photoconductive layer.

6. A method as recited in claim 5:

wherein said bias voltage comprises an alternating current (AC) voltage bias electrically coupled to the first plate and to the second plate, wherein the AC voltage bias generates an electric field between the first plate and the second plate; and

wherein said gradient in surface tension of said droplet is induced by illumination of at least a portion of said photoconductive layer.

7. A method as recited in claim 6, wherein the illuminated portion of the photoconductive layer exhibits lower electrical resistivity than non-illuminated portions thereby inducing a gradient in the electric field in a corresponding area adjacent to the photoconductive layer.

8. A method of manipulating a droplet, comprising:

providing a first plate and a second plate;

said first and second plates having opposing surfaces;

said first and second plates coupled to a voltage bias source;

wherein at least one of said opposing surfaces comprises a photoconductive layer;

placing a droplet between the opposing surfaces of the first and second plates; and

selectively illuminating portions of said photoconductive layer such that the illuminated portion induces a gradient in surface tension of the droplet;

wherein the gradient in the surface tension imparts motion to the droplet, thereby facilitating manipulation of the droplet.

9. A method as recited in claim 8, wherein said manipulation is selected from the group consisting essentially of dividing said droplet, joining said droplet with one or more other droplets, creating a droplet from a reservoir of liquid and moving said droplet from a first position to a second position.

10. A method as recited in claim 8, further comprising:

providing a plurality of spacers between said first and second plates thereby forming a cavity between said first and second plates;

wherein said droplet is positioned in said cavity for manipulation.

11. A method as recited in claim 8, wherein the illuminated portion of the photoconductive layer exhibits lower electrical resistivity than non-illuminated portions thereby inducing a gradient in the electric field in a corresponding area adjacent to the photoconductive layer.

12. A method of manipulating a droplet, comprising:

providing a first plate and a second plate electrically coupled to a bias voltage;

said first and second plates having opposing surfaces;

providing a source of light configured to impinge upon portions of said opposing surfaces of said first and second plates in a predetermined locations and sequence over time;

placing a plurality of droplets between the opposing surfaces of the first and second plates; and

selectively illuminating portions of at least one opposing surface of the first plate or the second plate according to the sequence such that the illuminated portion induces a gradient in surface tension of the droplets;

wherein the gradient in the surface tension imparts motion to the droplets, thereby facilitating manipulation of the droplets.

13. A method as recited in claim 12, wherein at least one of said opposing surfaces of said first and second plates comprises a photoconductive layer.

14. A method as recited in claim 12, wherein at least one of said opposing surfaces of said first and second plates has a hydrophobic layer.

15. A method as recited in claim 12, wherein said first plate is transparent and said opposing surface of said second plate comprises a photoconductive layer.

16. A method as recited in claim 12, wherein said first and second plates are illuminated by pulses of laser light.

17. A method as recited in claim 12, wherein said first and second plates are illuminated by a LED array.

18. A method as recited in claim 15, wherein said photoconductive layer of said second plate is illuminated by spots of light transmitted through said first plate.

19. A method as recited in claim 12:

wherein said bias voltage comprises an alternating current (AC) voltage bias electrically coupled to the first plate and to the second plate, wherein the AC voltage bias generates an electric field between the first plate and the second plate; and

wherein said gradient in surface tension of said droplet is induced by illumination of at least a portion of said photoconductive layer.