Method for making probe support and apparatus used for the method

• US 7,731,904 B2
• Filed: 09/17/2001
• Issued: 06/08/2010
• Est. Priority Date: 09/19/2000
• Status: Expired due to Fees

• Current Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Original Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Inventors: Suzuki, Tomohiro, Okamoto, Tadashi, Watanabe, Hidenori, Yamamoto, Nobuko
• Primary Examiner(s): FORMAN, BETTY J
• Application Number:
  US09/953,253
• Publication Number:
  US 20020182610A1
• Time in Patent Office: 3,186 Days
• Field of Search: 422/68.1, 422/100, 435/6, 435/174, 435/287.2, 536/23.1
• US Class Current: 422/504
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/0036 Nozzles
• CPC Class Codes: B01J 2219/00378 Piezoelectric or ink jet dispensers
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00529 DNA chips
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 3/0268 using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
• CPC Class Codes: B41J 2/14137 Resistor surrounding the nozzle opening
• CPC Class Codes: B41J 2002/1437 Back shooter
• CPC Class Codes: B41J 2002/14459 Matrix arrangement of the pressure chambers
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries

Abstract

A liquid discharging device includes a plurality of liquid discharge sections. Each liquid discharge section includes a reservoir, a nozzle that discharges a solution supplied from the reservoir, and discharge energy generating means that generates energy to discharge the solution from the nozzle. The number of the liquid discharge sections corresponds to the number of probe types to be formed. The nozzles are two-dimensionally arranged. Using this liquid discharging device, probe liquids are discharged from the corresponding reservoirs onto a solid-phase substrate to form a predetermined two-dimensional probe array of high-purity probes on the substrate. This process exhibits high reproducibility and processability, and the resulting probe array has high array density.

Claims

1. A liquid discharging device comprising:

a first substrate comprising a first surface having a plurality of energy generators for generating energy for discharging a liquid, and a second surface having a plurality of liquid supply openings arranged on the opposite side of said first surface, each supply opening corresponding to one of a plurality of first penetrating holes of the first substrate;

a nozzle member provided on said first surface side of said first substrate and having a plurality of liquid discharge nozzles; and

a second substrate laminated on said second surface side of said first substrate and having a plurality of second penetrating holes which continue through the second substrate,

wherein each one of said plurality of second penetrating holes is in communication with a supply opening of a corresponding one of said plurality of first penetrating holes, to form a plurality of individual flow paths that are independent from one another, each flow path comprising a single second penetrating hole, a single first penetrating hole, a single nozzle, and a single channel that communicates with the nozzle and the first penetrating hole,

wherein a position of the nozzle is not aligned with a position of the first penetrating hole,

and wherein each energy generator of the plurality of energy generators is positioned adjacent to a corresponding channel and a corresponding nozzle.

2. A liquid discharging device according to claim 1, wherein each of said plurality of first penetrating holes is shaped such that it narrows.

3. A liquid discharging device according to claim 1, wherein each of said plurality of first penetrating holes is shaped as a truncated quadrangular pyramid.

4. A liquid discharging device according to claim 1, wherein said plurality of discharge nozzles are arranged as lines and rows on a surface of said first substrate, and wherein a distance between lines of each of said plurality of liquid discharge nozzles is the same, and a distance between rows of each of said nozzles is the same within a substrate.

5. A liquid discharging device according to claim 1, wherein said plurality of discharge nozzles are arranged as lines and rows on a surface of said first substrate, and wherein a linear distance and a row distance between adjoining ones of said plurality of liquid discharge nozzles are the same within a substrate.

6. A liquid discharging device according to claim 1, wherein each of said plurality of energy generators is a piezo element.

7. A liquid discharging device according to claim 1, wherein each of said plurality of energy generators is a thermal energy generator.

8. A liquid discharging device according to claim 1, wherein said first substrate is silicon.

9. A liquid discharging device according to claim 8, wherein said plurality of first penetrating holes are formed by anisotropic etching of said silicon substrate using a tetramethylammonium hydroxide (TMAH) solution.

10. A liquid discharging device according to claim 1, wherein said first substrate is a silicon wafer and said second substrate is made of alumina or resin.

11. The liquid discharging device according to claim 1, wherein each of the plurality of discharge nozzles is formed in said first substrate to be within an area which is in line with a corresponding one of the plurality of supply openings.

12. A liquid discharging device according to claim 1, wherein the plurality of nozzles are arranged in lines and positions of discharge openings in adjacent lines are shifted by a predetermined pitch in a direction of the line.