Compact multi-wavelength optical reader and method of acquiring optical data on clustered assay samples using differing-wavelength light sources

• US 7,733,488 B1
• Filed: 01/28/2008
• Issued: 06/08/2010
• Est. Priority Date: 01/26/2007
• Status: Active Grant

• Current Assignee: Revolution Optics, LLC
• Original Assignee: Revolution Optics, LLC
• Inventors: Johnson, Lyle C.
• Primary Examiner(s): EVANS, FANNIE L
• Application Number:
  US12/020,900
• Time in Patent Office: 862 Days
• Field of Search: 356/414, 356/419, 356/420
• US Class Current: 356/414
• CPC Class Codes: G01J 3/02 Details
• CPC Class Codes: G01J 3/0229 using masks, aperture plates, spatial light modulators or spatial filters, e.g. reflective filters
• CPC Class Codes: G01N 21/0332 with temperature control control of temperature G05D23/00; cryostats F17C3/08
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 2201/0627 Use of several LED's for spectral resolution

Abstract

An optical reader having an array of differing-color light sources and a controller for controlling the light sources and acquisition of optical data. The light sources are arranged, and the controller is configured, to allow rapid acquisition of optical data regarding individual sample wells of a cluster of such wells. In some embodiments, multiple ones of the differing-color light sources are illuminated simultaneously for acquiring optical data on a corresponding number of sample wells. Depending on the configuration of the array and number of differing-color light sources illuminated simultaneously, the optical reader can acquire optical data for several wavelengths in a fraction of the time of conventional optical readers. Other embodiments include one or more non-contact temperature sensors for acquiring temperature data substantially simultaneously with the optical data. The temperature data can be used, for example, to adjust the optical data or warn a user of out-of-specification temperature conditions.

Claims

1. An optical reader for acquiring optical data corresponding to each of a plurality of sample wells of a cluster of sample wells, the optical reader comprising:

an illuminating array including a plurality of light sources of predetermined differing-wavelength outputs;

a detector operatively configured and located to detect light from said predetermined differing-wavelength outputs from said illuminating array;

a reading region located between said illuminating array and said detector during operation of the optical reader; and

a controller operatively configured to control movement of one, the other, or both, of said illuminating array and the cluster of sample wells and to control pulsing of said plurality of light sources so as to illuminate simultaneously corresponding respective ones of the plurality of sample wells with said predetermined differing-wavelength outputs.

2. An optical reader according to claim 1, wherein said plurality of light sources includes a plurality of differing output color light emitting diodes arranged in a planar array.

3. An optical reader according to claim 1, wherein said illuminating array and said detector are configured to allow a user to obtain light-absorbance data during use of the optical reader.

4. An optical reader according to claim 1, wherein the cluster of sample wells is defined by a microplate.

5. An optical reader according to claim 1, wherein said plurality of light sources further includes a plurality of filters corresponding respectively to said plurality of light sources, said plurality of light filters working in conjunction with said plurality of light sources to provide said predetermined differing wavelength outputs.

6. An optical reader according to claim 1, wherein said plurality of light sources includes at least three light sources having corresponding respective output colors differing from one another.

7. An optical reader according to claim 6, wherein said controller is operatively configured to simultaneously pulse said at least three light sources when said at least three light sources are located proximate corresponding respective differing ones of the plurality of sample wells.

8. An optical reader according to claim 1, said plurality of light sources are arranged into a plurality of sub-arrays each containing only ones of said plurality of light sources of a common wavelength output, said common wavelength output differing as between ones of said plurality of sub-arrays.

9. An optical reader according to claim 8, wherein each of said plurality of sub-arrays is a one-dimensional array.

10. An optical reader according to claim 8, wherein each of said plurality of sub-arrays is a multi-dimensional array.

11. An optical reader according to claim 10, wherein the optical reader is configured for acquiring optical data regarding sample wells of microplates having differing well spacings and at least one of said sub-arrays contains ones of said plurality of light sources arranged to accommodate the differing well spacings.

12. An optical reader according to claim 8, wherein the plurality of sample wells are arranged in rows in the cluster and each of said plurality of sub-arrays includes at least a number of said plurality of light sources that corresponds to the number of the sample wells in any of the rows in the cluster.

13. An optical reader according to claim 8, wherein said plurality of sub-arrays includes at least three sub-arrays having corresponding respective color outputs differing from one another, said controller operatively configured to simultaneously pulse at least one of said plurality of light sources in each of said at least three sub-arrays when each of said at least one of said plurality of light sources is located proximate a corresponding one of the plurality of sample wells.

14. An optical reader according to claim 13, wherein said controller is operatively configured to simultaneously pulse multiple ones of said plurality of light sources in each of said at least three sub-arrays when each of said multiple ones of said plurality of light sources is located proximate a corresponding one of the plurality of sample wells.

15. An optical reader according to claim 1, wherein said plurality of light sources are arranged into a first arrangement and said detector comprises an array of photosensors arranged into a second arrangement substantially the same as said first arrangement.

16. A method of acquiring optical data corresponding to each of a plurality of sample wells of a cluster of sample wells, the method comprising:

positioning the cluster of sample wells in a reading region located between a light-source array and a light detector during reading, the light-source array including at least two light sources having differing output wavelengths and located relative to one another so that when the cluster is in a reading position within the reading region, the at least two light sources are located proximate corresponding respective ones of the plurality of sample wells;

when the at least two light sources are located adjacent corresponding respective ones of the plurality of sample wells, simultaneously pulsing the at least two light sources so as to direct light toward a first side of the cluster;

in coordination with the simultaneous pulsing, sensing via the detector, from a second side of the cluster opposite the first side, light from the at least two light sources that passed through the corresponding respective ones of the plurality of wells; and

acquiring optical data corresponding to the light detected by the detector.

17. A method according to claim 16, wherein the light-source array includes three light sources having differing output wavelengths and located relative to one another so that when the cluster is in a reading position within the reading region, the three light sources are located proximate corresponding respective ones of the plurality of sample wells and the method includes, when the three light sources are located adjacent corresponding respective ones of the plurality of sample wells, simultaneously pulsing the three sources so as to direct light toward the first side of the cluster.

18. A method according to claim 16, wherein the light-source array includes at least two sub-arrays each containing a plurality of like-wavelength output light sources having a primary wavelength, the primary wavelength differing as between the at least two sub-arrays, the method including, when ones of the plurality of like-wavelength output light sources in each of the at least two sub-arrays are located proximate corresponding respective ones of the plurality of wells, simultaneously pulsing ones of the plurality of like-wavelength output light sources in all of the at least two sub-arrays.

19. A method according to claim 18, wherein each of the at least two sub-arrays is a linear array and the method further includes pulsing alternating ones of the plurality of like-wavelength output light sources in each linear array.

20. A method according to claim 16, wherein said acquiring of optical data includes acquiring light-absorbance data.