Methods for altering cell fate
First Claim
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1. A method of altering gene expression of a mammalian fibroblast, said method comprising incubating a permeabilized fibroblast with an activated T-cell extract, said permeabilized fibroblast having pores in its plasma membrane or a partial plasma membrane, wherein said incubation results in an alteration of gene expression in said fibroblast, wherein said fibroblast with altered gene expression expresses IL-2 receptor.
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Abstract
The invention provides methods for altering the expression profile of a cell to convert the cell from one cell type to a desired cell type. These reprogrammed cells may be used in a variety of medical applications for treating a mammal in need of a particular cell type.
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Citations
22 Claims
- 1. A method of altering gene expression of a mammalian fibroblast, said method comprising incubating a permeabilized fibroblast with an activated T-cell extract, said permeabilized fibroblast having pores in its plasma membrane or a partial plasma membrane, wherein said incubation results in an alteration of gene expression in said fibroblast, wherein said fibroblast with altered gene expression expresses IL-2 receptor.
- 8. A method of altering gene expression of a mammalian fibroblast, said method comprising incubating a permeabilized mammalian fibroblast with an interphase cell extract from an embryonic stem cell, said permeabilized mammalian fibroblast having pores in its plasma membrane or a partial plasma membrane, wherein said incubation results in an alteration of gene expression in said mammalian fibroblast, wherein said fibroblast with altered gene expression expresses Oct4 or alkaline phosphatase.
- 15. A method of altering gene expression of a mammalian fibroblast, said method comprising incubating a permeabilized mammalian fibroblast with an interphase cell extract from a neural cell, said permeabilized mammalian fibroblast having pores in its plasma membrane or a partial plasma membrane, wherein said incubation results in an alteration of gene expression in said mammalian fibroblast, wherein said fibroblast with altered gene expression expresses neurofilament protein NF200.
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