Method and device for 3 dimensional imaging of suspended micro-objects providing high-resolution microscopy

• US 7,738,695 B2
• Filed: 10/24/2003
• Issued: 06/15/2010
• Est. Priority Date: 10/25/2002
• Status: Expired due to Fees

• Current Assignee: Institut Pasteur, Perkinelmer Cellular Technologies Germany GmbH (Revvity, Inc.)
• Original Assignee: Institut Pasteur, Perkinelmer Cellular Technologies Germany GmbH (Revvity, Inc.)
• Inventors: Schnelle, Thomas, Muller, Torsten, Shorte, Spencer
• Primary Examiner(s): Tucker; Wesley
• Assistant Examiner(s): Bitar; Nancy
• Application Number:
  US10/532,147
• Publication Number:
  US 20060126921A1
• Time in Patent Office: 2,426 Days
• Field of Search: 382/154, 382/129, 382/285, 359/368, 359/676, 359/738, 436/524, 436/526, 435/7.1, 219/121.68, 210/645, 210/732, 345/418, 345/419, 345/420
• US Class Current: 382/154
• CPC Class Codes: G01N 2015/144 Imaging characterised by its optical setup
• CPC Class Codes: G01N 2015/1445 Three-dimensional imaging, imaging in different image planes, e.g. under different angles or at different depths, e.g. by a relative motion of sample and detector, for instance by tomography
• CPC Class Codes: G01N 2015/1452 Adjustment of focus; Alignment
• CPC Class Codes: G02B 21/22 Stereoscopic arrangements
• CPC Class Codes: G02B 21/367 providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison

Abstract

A method for high-resolution image recording of at least one object with a microscope, includes the steps of: (a) positioning the object in a receptacle being arranged in the optical axis of the microscope, (b) generating at least two first data sets per object which represent intermediate images of the object with at least two different orientations relative to the optical axis of the microscope, wherein the different orientations of the object are provided by moving the object relative to the receptacle, and (c) evaluating the data sets for obtaining quantitative three dimensional information.

Claims

1. A method for high-resolution image recording of at least one object with a microscope, comprising the steps of:

positioning the at least one object in a receptacle arranged in an optical axis of the microscope,

generating at least two first data sets per object, wherein;

(a) the at least two first data sets represent intermediate images of the at least one object with at least two different predetermined orientations relative to the optical axis of the microscope, (b) the at least two different predetermined orientations of the object are provided by controlled movement of the at least one object relative to the receptacle, and (c) the controlled movement comprises a rotation of the at least one object by an influence of electrical field forces, said object being rotated around at least one of a predetermined axis and a predetermined rotation angle, and

evaluating the data sets to obtain quantitative three-dimensional information of the at least one object, wherein said quantitative three-dimensional information represents a three-dimensional shape of the at least one object.

2. The method according to claim 1, wherein said moving of the at least one object relative to the receptacle further comprises a translation of the at least one object by the influence of the electric field forces.

3. The method according to claim 2, wherein said rotation comprises at least one rotation with a rotation axis parallel to the optical axis.

4. The method according to claim 2, wherein said rotation comprises at least one rotation with a rotation axis slanted relative to the optical axis.

5. The method according to claim 4, wherein said rotation axis is slanted within an angle range of up to 90°

.

6. The method according to claim 2, wherein said rotation comprises:

a rotation in a continuous mode or for predetermined time periods and angles, and/or

a rotation with changing rotational axes.

7. The method according to claim 2, wherein said rotation is conducted by holding the at least one object at a fixed position by said electric field forces and by rotating the at least one object by optical forces.

8. The method according to claim 1, further comprising steps of generating further intermediate images of the object, each with another focal plane, respectively, wherein each said focal plane is adjusted by scanning an objective of the microscope parallel to the optical axis.

9. The method according to claim 8, wherein said at least two different predetermined orientations of the object and said scanning an objective are conducted in an alternating mode.

10. The method according to claim 1, wherein said positioning comprises suspending said at least one object in a liquid in said receptacle.

11. The method according to claim 1, wherein said step of evaluating the data sets comprises at least one step selected from the group consisting of removing out-of-focus light and reconstructing a three-dimensional map/image of the at least one object.

12. The method according to claim 1, wherein said at least one object comprises at least one eukaryotic cell, at least one prokaryotic cell and/or at least one artificial particle.

13. The method according to claim 1, wherein said microscope is used as a fluorescence microscope, a phase contrast microscope, a differential interference contrast microscope or a confocal microscope.

14. An imaging device for high-resolution image recording of at least one object, comprising:

a microscope imaging system with an optical axis,

a receptacle for accommodating said at least one object, said receptacle being arranged in said optical axis, and

a control circuit being arranged for;

(a) generating at least two first data sets per object, wherein said at least two first data sets represent intermediate images of the at least one object with at least two different predetermined orientations relative to the optical axis, (b) providing controlled movement of the at least one object relative to the receptacle to generate the at least two different predetermined orientations, wherein the controlled movement comprises a rotation of the at least one object by an influence of electrical field forces, said object being rotated around at least one of a predetermined axis and a predetermined rotation angle, and (c) evaluating the data sets to obtain an object image representing a three-dimensional shape of the at least one object, and

a driving device adapted to controllably move the at least one object relative to the receptacle.

15. The imaging device according to claim 14, wherein said receptacle comprises a chamber of a fluidic microsystem and said driving device comprises microelectrodes arranged at walls of said chamber and connected with said control circuit.

16. The imaging device according to claim 15, wherein said driving device comprises at least three microelectrodes arranged in one plane in said chamber.

17. The imaging device according to claim 16, wherein said driving device comprises at least six microelectrodes arranged in two planes in said chamber.

18. The imaging device according to claim 14, wherein said control circuit comprises a switching box arranged for switching a rotation axis of the at least one object.

19. A method for high-resolution image recording of at least one object with a measuring device with a predetermined measurement field, comprising the steps of:

positioning the at least one object in a receptacle arranged in the measurement field of the measuring device,

generating at least two first data sets per object, wherein;

(a) the at least two first data sets represent intermediate data of the at least one object with at least two different predetermined orientations relative to the measurement field of the measuring device, (b) the at least two different predetermined orientations of the object are provided by controlled movement of the at least one object relative to the receptacle, and (c) the controlled movement comprises a rotation of the at least one object by an influence of electrical field forces, said object being rotated around at least one of a predetermined axis and a predetermined rotation angle, and

evaluating the data sets to obtain quantitative three-dimensional information of the at least one object, wherein said quantitative three-dimensional information represents the three-dimensional shape of the at least one object.

20. The method according to claim 19, wherein said measuring device comprises a microscope and said measurement field is an optical axis of the microscope.

21. The method according to claim 19, wherein said measuring device comprises an impedance measurement device and said measurement field is the receptacle.