Optically decodable microcarries, arrays and methods
First Claim
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1. A hybridization assay for at least one of a multiplicity of nucleic acid sequences in an analyte comprising the steps of:
- (a) contacting said analyte with a mixture of encoded microcarriers having immobilized on their surfaces(i) a hybridization probe for one of said multiplicity of sequences, whose hybridization to said at least one sequence can be detected, and(ii) a coding scheme comprising a plurality of signaling hairpins that are not hybridization probes for said multiplicity of sequences, including said at least one sequence, comprising quenched, fluorophore-labeled hairpin molecules each comprising an interacting affinity pair separated by a linking moiety, one member of said affinity pair having bound thereto at least one quenched fluorophore, wherein interaction of the affinity pair is disruptable to unquench said at least one fluorophore by a physical or chemical change in a condition of its environment, wherein the disruption of the interaction of at least one affinity pair occurs at a first level of said condition and the disruption of the interaction of at least another affinity pair occurs at a second level of said condition, and wherein said disruptions are optically differentiable, and wherein the coding scheme for identifying individual microcarriers in said mixture comprises a combination of multiple spectrally differentiable fluorophores and multiple affinity pairs disruptable at detectably different levels of said condition;
(b) forming a distributed array of said microcarriers wherein location of said microcarriers in said distributed array is not used to identify said at least one nucleic acid sequence;
(c) determining which microcarriers have hybridization probes hybridized to said at least one nucleic acid sequence of said analyte; and
(d) optically decoding the microcarriers having said at least one nucleic acid sequence hybridized to its hybridization probes to identify said at least one nucleic and sequence by changing said condition to said detectably different levels to disrupt quenching, and detecting changes in fluorescence from the signaling hairpins.
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Abstract
A coding scheme for microcarriers suitable for use in distributed arrays includes labeling the carriers with quenched signaling hairpin molecules with any one of three to eight distinguishable fluorophores wherein the hairpins are of at least two types, most preferably three types, that open and fluoresce differentially as a chemical or physical condition, for example temperature, is changed. Mixtures of microcarriers having immobilized capture probes can be decoded by measuring fluorescence from said fluorophores under conditions under which only one type of hairpin is open, under which two types of hairpin are open, and so on. Mixtures of coded microcarriers with capture probes are used in assays for nucleic acids utilizing microarray methods.
10 Citations
22 Claims
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1. A hybridization assay for at least one of a multiplicity of nucleic acid sequences in an analyte comprising the steps of:
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(a) contacting said analyte with a mixture of encoded microcarriers having immobilized on their surfaces (i) a hybridization probe for one of said multiplicity of sequences, whose hybridization to said at least one sequence can be detected, and (ii) a coding scheme comprising a plurality of signaling hairpins that are not hybridization probes for said multiplicity of sequences, including said at least one sequence, comprising quenched, fluorophore-labeled hairpin molecules each comprising an interacting affinity pair separated by a linking moiety, one member of said affinity pair having bound thereto at least one quenched fluorophore, wherein interaction of the affinity pair is disruptable to unquench said at least one fluorophore by a physical or chemical change in a condition of its environment, wherein the disruption of the interaction of at least one affinity pair occurs at a first level of said condition and the disruption of the interaction of at least another affinity pair occurs at a second level of said condition, and wherein said disruptions are optically differentiable, and wherein the coding scheme for identifying individual microcarriers in said mixture comprises a combination of multiple spectrally differentiable fluorophores and multiple affinity pairs disruptable at detectably different levels of said condition; (b) forming a distributed array of said microcarriers wherein location of said microcarriers in said distributed array is not used to identify said at least one nucleic acid sequence; (c) determining which microcarriers have hybridization probes hybridized to said at least one nucleic acid sequence of said analyte; and (d) optically decoding the microcarriers having said at least one nucleic acid sequence hybridized to its hybridization probes to identify said at least one nucleic and sequence by changing said condition to said detectably different levels to disrupt quenching, and detecting changes in fluorescence from the signaling hairpins. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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Specification
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Current AssigneeRutgers University
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Original AssigneeThe Public Health Research Institute of the City of New York, Inc.
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InventorsKramer, Fred R., Marras, Salvatore A. E., Tyagi, Sanjay, Trunfio, Hiyam Elhajj
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Primary Examiner(s)CALAMITA, HEATHER
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Application NumberUS10/791,502Publication NumberTime in Patent Office2,303 DaysField of SearchNoneUS Class Current435/6.11CPC Class CodesC12Q 1/6818 involving interaction of tw...C12Q 2523/113 Denaturating agentsC12Q 2525/301 Hairpin oligonucleotidesC12Q 2527/107 Temperature of melting, i.e...C12Q 2565/107 Alteration in the property ...
