Sequential analysis of biological samples
First Claim
1. A method of probing multiple targets in a biological sample comprising:
- (a) providing a biological sample containing multiple targets(b) binding at least one fluorescent probe to one or more targets present in the sample;
(c) binding at least one control probe to one or more targets present in the sample;
(d) observing a signal from the fluorescent probe bound in step (b) and a control signal from the control probe bound in step (c);
(e) applying to the sample in step (d) a solution comprising an oxidizing agent that selectively inactivates the fluorescent probe and not the control probe;
(f) binding at least one fluorescent probe to one or more targets present in the sample of step (e); and
(g) observing a signal from the fluorescent probe bound in step (f).
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Accused Products
Abstract
Methods for probing multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets, binding at least one fluorescent probe to one or more target present in the sample, and binding at least at least one control probe to one or more target present in the sample. The methods include the steps of observing a signal from the fluorescent probe and a control signal from the control probe and applying to the sample a basic solution containing an oxidizing agent that selectively inactivates the fluorescent probe and not the control probe. The methods further include the steps of binding at least one fluorescent probe to one or more target present in the sample and observing a signal from the fluorescent probe. The methods disclosed herein also provide for multiple iterations of binding, observing, and oxidizing for deriving information about multiple targets in a single sample. An associated kit is also provided.
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Citations
24 Claims
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1. A method of probing multiple targets in a biological sample comprising:
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(a) providing a biological sample containing multiple targets (b) binding at least one fluorescent probe to one or more targets present in the sample; (c) binding at least one control probe to one or more targets present in the sample; (d) observing a signal from the fluorescent probe bound in step (b) and a control signal from the control probe bound in step (c); (e) applying to the sample in step (d) a solution comprising an oxidizing agent that selectively inactivates the fluorescent probe and not the control probe; (f) binding at least one fluorescent probe to one or more targets present in the sample of step (e); and (g) observing a signal from the fluorescent probe bound in step (f). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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23. A method of probing multiple targets in a biological sample comprising:
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(a) providing a biological sample containing multiple targets (b) binding at least one fluorescent probe to one or more targets present in the sample; (c) binding at least one control probe to one or more targets present in the sample; (d) observing a signal from the fluorescent probe bound in step (b) and a control signal from the control probe bound in step (c); (e) applying to the sample in step (d) a basic solution comprising an oxidizing agent that selectively inactivates the fluorescent probe and not the control probe; (f) binding at least one fluorescent probe to one or more targets present in the sample of step (e); (g) observing a signal from the fluorescent probe bound in step (f); and (h) co-localizing at least two targets in the sample. - View Dependent Claims (24)
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Specification