Cellulases and their uses

• US 7,741,093 B2
• Filed: 04/29/2005
• Issued: 06/22/2010
• Est. Priority Date: 04/29/2005
• Status: Active Grant

• Current Assignee: AB Enzymes Oy (Garfield Weston Foundation)
• Original Assignee: AB Enzymes Oy (Garfield Weston Foundation)
• Inventors: Paloheimo, Marja, Ojapalo, Pentti, Alapuranen, Marika, Puranen, Terhi, Valtakari, Leena, Kallio, Jarno, Vehmaanpera, Jari
• Primary Examiner(s): RAGHU, GANAPATHIRAM
• Application Number:
  US11/119,526
• Publication Number:
  US 20060246566A1
• Time in Patent Office: 1,880 Days
• Field of Search: None
• US Class Current: 435/209
• CPC Class Codes: C12N 9/2437 Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
• CPC Class Codes: C12Y 302/01004 Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
• CPC Class Codes: C12Y 302/01091 Cellulose 1,4-beta-cellobiosidase (3.2.1.91)

Abstract

The present invention provides novel cellulase fusion proteins, preparations of cellulase fusion proteins and compositions of cellulase fusion proteins. The present invention further provides cellulase expression vectors, host cells expressing cellulase and methods for preparing such vectors and cells. Uses of cellulases, cellulase preparations and cellulase compositions in the textile, detergent, pulp and paper industries are also provided.

Claims

1. A cellulase fusion protein comprising:

a first amino acid sequence of SEQ ID NO;

2 or a biologically active fragment thereof from the 20K cellulase of Melanocarpus albomyces, wherein the first amino acid sequence has a cellulase activity;

a second amino acid sequence of a linker and cellulose binding domain (CBD) of Trichoderma reesei cellobiohydrolase I from SEQ ID NO;

4 or a biologically active fragment thereof, wherein the second amino acid sequence has a CBD activity; and

a junction region having the following formula;

¹Val-²Gln-³Ile-⁴Pro-⁵Ser-⁶Ser 

 

(SEQ ID NO;

     52)

between said first amino acid sequence and said second amino acid sequence, whereby a stable fusion protein is obtained.

2. An enzyme preparation comprising the cellulase fusion protein of claim 1.

3. A detergent composition comprising the cellulase fusion protein of claim 1 and one or more auxiliaries.

4. The detergent composition of claim 3, wherein said auxiliaries are selected from the group consisting of surface active agents, surfactants, bleaching agents and builders.

5. A cellulase fusion protein comprising:

a first amino acid sequence of a cellulase core encoded by the nucleotide sequence of SEQ ID NO;

1 or a fragment thereof long enough to encode a biologically active first polypeptide, wherein the first amino acid sequence or polypeptide has a cellulase activity,

a second amino acid sequence of a linker and a cellulose binding domain CBD of Trichoderma reesei cellobiohydrolase I encoded by the nucleotide sequence of SEQ ID NO;

3 or a fragment thereof long enough to encode a biologically active second polypeptide, wherein the second amino acid sequence or polypeptide has a CBD activity, and

a junction region between said first amino acid sequence and said second amino acid sequence or polypeptide, whereby a stable fusion protein is obtained.

6. An enzyme preparation comprising the cellulase fusion protein of claim 5.

7. A detergent composition comprising the cellulase fusion protein of claim 5 and one or more auxiliaries.

8. The detergent composition of claim 7, wherein said auxiliaries are selected from the group consisting of surface active agents, surfactants, bleaching agents and builders.