Optical interrogation system and method for using same

• US 7,741,598 B2
• Filed: 03/12/2009
• Issued: 06/22/2010
• Est. Priority Date: 08/01/2007
• Status: Expired due to Fees

• Current Assignee: Corning Incorporated
• Original Assignee: Corning Incorporated
• Inventors: Modavis, Robert A., Wu, Qi, Pastel, David A., Caracci, Stephen J.
• Primary Examiner(s): Le; Que T
• Assistant Examiner(s): BENNETT, JENNIFER D
• Application Number:
  US12/402,901
• Publication Number:
  US 20090219540A1
• Time in Patent Office: 467 Days
• Field of Search: 250/201.2, 250/458.1, 250/216, 25022714-22724, 250/227.11, 250/222.1, 250/578.1, 250/221, 436167-172, 435/7.1, 435/287.2, 435/7.9, 435/283.1, 356/445, 356/600, 356/601, 356319-334, 356/246, 422/68.1, 422 8205- 8209, 422/82.11
• US Class Current: 250/227.11
• CPC Class Codes: G01N 2021/4742 comprising optical fibres
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/39 using tunable lasers
• CPC Class Codes: G01N 21/7743 the reagent-coated grating coupling light in or out of the waveguide
• CPC Class Codes: G01N 2201/08 Optical fibres; light guides

Abstract

An optical interrogation system and a method are described herein that enable the interrogation of one or more biosensors which can be located within the wells of a microplate. In one embodiment, the optical interrogation system has a tunable laser, N-fiber launches, N-lenses and N-detectors that are set-up to interrogate N-biosensors. In another embodiment, the optical interrogation system has a tunable laser, N-fiber launches, N+1 lenses and N-detectors that are set-up to interrogate N-biosensors.

Claims

1. An optical interrogation system comprising:

a tunable laser for generating an optical beam;

a first fiber launch for receiving the optical beam generated by said tunable laser and then outputting a first optical beam;

a first lens for collimating the first optical beam outputted from said first fiber launch such that the collimated optical beam illuminates a first biosensor;

said first lens for receiving a first reflected optical beam from the first biosensor;

a first detector, located ahead or past a focal point of said first lens, for receiving a first defocused optical beam from said first lens; and

a data processing device for receiving one or more intensity spot patterns associated with the first defocused optical beam received at said first detector.

2. The optical interrogation system of claim 1, further comprising:

a second fiber launch for receiving the optical beam generated by said tunable laser and then outputting a second optical beam;

a second lens for collimating the second optical beam outputted from said second fiber launch such that the collimated second optical beam illuminates a second biosensor;

said second lens for receiving a second reflected optical beam from the second biosensor;

a second detector, located ahead or past a focal point of said second lens, for receiving the second defocused optical beam from said second lens; and

said data processing device for receiving one or more intensity spot patterns associated with the second defocused optical beam received at said second detector.

3. The optical interrogation system of claim 1, further comprising a wavelength tracking device which tracks the distinct wavelengths of the optical beam generated by said tunable laser.

4. The optical interrogation system of claim 1, further comprising a power tracking device which receives the optical beam generated by said tunable laser and tracks the power of said tunable laser.

5. The optical interrogation system of claim 1, wherein said data processing device further processes the one or more intensity spot patterns to determine whether or not there was a biochemical interaction on the biosensor.

6. The optical interrogation system of claim 1, wherein:

said first biosensor is located within a first well of a microplate; and

said first biosensor has a mask located on a surface thereon to separate a signal region from a reference region.

7. A method for interrogating one or more biosensors, said method comprising the steps of:

emitting a first optical beam which has a predetermined sequence of distinct wavelengths over a predetermined time period;

using a first lens to collimate the first emitted optical beam such that the first collimated optical beam illuminates a first biosensor;

using said first lens to receive a first reflected optical beam from the first biosensor;

using a first detector to receive the first reflected optical beam from said first lens, where the first detector is positioned ahead or past a focal point of said first lens; and

using a data processing device to receive a plurality of intensity spot patterns associated with the first reflected optical beam that was received at said first detector where each intensity spot pattern corresponds with one of the distinct wavelengths of the first emitted optical beam.

8. The method of claim 7, further comprising the steps of:

emitting a second optical beam which has a predetermined sequence of distinct wavelengths over a predetermined time period;

using a second lens to collimate the second emitted optical beam such that the second collimated optical beam illuminates a second biosensor;

using said second lens to receive a second reflected optical beam from the second biosensor;

using a second detector to receive the second reflected optical beam from said second lens, where the second detector is positioned ahead or past a focal point of said second lens; and

using a data processing device to receive a plurality of intensity spot patterns associated with the second reflected optical beam that was received at said second detector where each intensity spot pattern corresponds with one of the distinct wavelengths of the second emitted optical beam.

9. The method of claim 7, further comprising a step of tracking the distinct wavelengths of the first emitted optical beam.

10. The method of claim 7, further comprising a step of tracking the power of a tunable laser which generated the first emitted optical beam.

11. The method of claim 7, further comprising a step of using the data processing device to process the intensity spot patterns to determine whether or not there was a biochemical interaction on the first biosensor.

12. The method of claim 7, wherein:

said first biosensor is located within a first well of a microplate; and

said first biosensor has a mask located on a surface thereon to separate a signal region from a reference region.