Molecular modification assays
First Claim
Patent Images
1. A method of detecting nucleotide cyclization or decyclization in a sample, comprising:
- contacting a nucleotide with a binding partner that binds specifically to a noncyclized form but not to a cyclized form of the nucleotide, the binding partner including at least one metal ion required for specific binding of the binding partner to the noncyclized form of the nucleotide;
contacting the nucleotide with at least one enzyme selected from the group consisting of cyclases and phosphodiesterases;
detecting a response indicative of the extent of binding between the noncyclized form of the nucleotide and the binding partner; and
correlating the response with a cyclization or decyclization activity of the at least enzyme on the nucleotide.
3 Assignments
0 Petitions
Accused Products
Abstract
Assays for detecting molecular modifications such as phosphate modifications and the presence and/or activity of enzymes and other agents involved in facilitating or otherwise regulating such modifications.
321 Citations
25 Claims
-
1. A method of detecting nucleotide cyclization or decyclization in a sample, comprising:
-
contacting a nucleotide with a binding partner that binds specifically to a noncyclized form but not to a cyclized form of the nucleotide, the binding partner including at least one metal ion required for specific binding of the binding partner to the noncyclized form of the nucleotide; contacting the nucleotide with at least one enzyme selected from the group consisting of cyclases and phosphodiesterases; detecting a response indicative of the extent of binding between the noncyclized form of the nucleotide and the binding partner; and correlating the response with a cyclization or decyclization activity of the at least enzyme on the nucleotide. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
-
Specification