Digital cell

• US 7,747,390 B2
• Filed: 06/25/2004
• Issued: 06/29/2010
• Est. Priority Date: 06/25/2003
• Status: Expired due to Fees

• Current Assignee: National Institute of Advanced Industrial Science and Technology (Government of Japan)
• Original Assignee: National Institute of Advanced Industrial Science and Technology (Government of Japan)
• Inventors: Miyake, Jun, Yoshikawa, Tomohiro, Miyake, Masato
• Primary Examiner(s): Brusca; John S
• Application Number:
  US10/562,469
• Publication Number:
  US 20060253258A1
• Time in Patent Office: 2,195 Days
• Field of Search: None
• US Class Current: 702/19
• CPC Class Codes: C12M 41/48 Automatic or computerized control automatic analysis G01N35/00
• CPC Class Codes: G01N 33/5005 involving human or animal cells immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025

Abstract

It is intended to provide a method and system for carrying out data production with respect to the actual status of cells as a profile. It is also intended to provide a system and method for presenting time-lapse and/or real-time information of cell interior directly, or as it is, from the viewpoint of a complex system. It is further intended to provide a method of presenting a digital cell. Thus, there is provided a method of producing profile data relating to cell information, comprising the step (a) of arranging cells in an immobilized form on a support and the step (b) of monitoring over time biological factors on or inside the cells or an aggregate thereof, thereby producing profile data for the cells. Furthermore, there is provided a method of producing a digital cell, comprising procuring experimental data by the use of the above method.

Claims

1. An apparatus for producing a digital cell, comprising:

a) means for obtaining a cell parameter specifying a cell of experimental interest;

b) means for obtaining an environment parameter specifying environment under which the cell specified by the cell parameter is cultured;

c) means for obtaining a stimulus parameter specifying a stimulus to be given to the cell specified by the cell parameter;

d) means for obtaining a stimulus response result showing a result which the cell specified by the cell parameter responds to the stimulus specified by the stimulus parameter under the environment specified by the environment parameter wherein the stimulus response comprises profile data for the cell obtained by monitoring a biological agent or a collection thereof on or in the cell over time, and wherein during the monitoring, the cell is immobilized to a support by a composition comprising a salt, and a 29 kDa fragment of fibronectin;

e) means for producing an experimental data against the cell, by correlating the cell parameter, the environment parameter, the stimulus parameter and the stimulus response result; and

f) means for providing at least one collection of experimental data as a digital cell, by optionally repeating steps performed by the means a) through e) to produce at least one collection of experimental data against the cell,

wherein the apparatus further comprises a means for storing the at least one collection of experimental data against the cell.

2. A computer system for providing a service which reproduces an experimental result on an actual cell using a digital cell produced by the apparatus of claim 1, comprising:

a service requester being composed such that it can have access to a database having at least one digital cell stored thereon, each of the at least one digital cell is expressed as a collection of at least one experimental data on a cell of experimental interest, wherein each of the at least one experimental data comprises a cell parameter specifying the cell, an environment parameter specifying an environment under which the cell specified by the cell parameter is culture, a stimulus parameter specifying a stimulus to be given to the cell specified by the cell parameter, and a stimulus response result showing a result which the cell specified by the cell parameter responds to the stimulus specified by the stimulus parameter under the environment specified by the environment parameter; and

a service provider requesting a service desired by a user;

wherein the service requester comprises;

means for receiving the cell parameter, the environment parameter and the stimulus parameter to produce a request comprising the cell parameter, the environment parameter and the stimulus parameter; and

means for providing the request to the service provider, and

wherein the service provider comprises;

means for searching the database in response to the request by the service provider to determine whether or not there is the stimulus response result relating to the cell parameter, the environment parameter and the stimulus parameter included in the request in the database; and

means for providing the stimulus response result to the service requester by the service provider, when determined that there exists the stimulus response result relating to the cell parameter, the environment parameter and the stimulus parameter included in the request in the database;

wherein the service requester further comprises

means for presenting the stimulus response result by the service requester.

3. A computer system according to claim 2 wherein the service requester is a Web browser which the user operates, the service provider is a Web server linked to the service requester via the Internet.

4. A computer system according to claim 2, wherein the service requester provides the request to the service provider in a format described in the XML language.

5. A computer system according to claim 2, wherein the service provider provides the stimulus response result to the service requester in a format described in the XML language.

6. A computer system for providing a service which reproduces an experimental result on an actual cell using a digital cell produced by the apparatus of claim 1, comprising:

a plurality of service providers, each composed such that the plurality of service providers can have access to a database having at least one digital cell stored thereon, each of the at least one digital cell is expressed as a collection of at least one experimental data on a cell of experimental interest, wherein each of the at least one experimental data comprises a cell parameter specifying the cell, an environment parameter specifying an environment under which the cell specified by the cell parameter is cultured a stimulus parameter specifying a stimulus to be given to the cell specified by the cell parameter, and a stimulus response result showing a result which the cell specified by the cell parameter responds to the stimulus specified by the stimulus parameter under the environment specified by the environment parameter;

a service registry which stores at least one service which the plurality of service providers can provide; and

a service provider requesting a service desired by a user;

wherein the service requester comprises;

means for receiving the cell parameter, the environment parameter and the stimulus parameter to produce a request comprising the cell parameter, the environment parameter and the stimulus parameter;

means for searching the service registry in response to the request by the service requester to determine whether or not there exists a service provider capable of providing a service of the request amongst the plurality of service providers and

means for providing the request to the service provider by the service requester when determined that there exists a service provider capable of providing a service of the request amongst the plurality of service providers,

wherein each of the plurality of service providers comprises;

means for searching the database in response to the request by the service provider to determine whether or not there is the stimulus response result relating to the cell parameter, the environment parameter and the stimulus parameter included in the request in the database; and

means for providing the stimulus response result to the service requester by the service provider, when determined that there exists the stimulus response result relating to the cell parameter, the environment parameter and the stimulus parameter included in the request in the database;

wherein the service requester further comprises

means for presenting the stimulus response result by the service requester.

7. A computer system according to claim 6, wherein the service requester is a Web server connected to a Web browser which the user operates via the Internet, each of the plurality of service providers is a Web server connected to the service requester via the Internet.

8. A computer system according to claim 6, wherein the service requester provides the request to the service provider in a format described in the XML language.

9. A computer system according to claim 6, wherein the service provider provides the stimulus response result to the service requester in a format described in the XML language.