Optical assay system for intraoperative assessment of tumor margins

• US 7,751,039 B2
• Filed: 03/29/2007
• Issued: 07/06/2010
• Est. Priority Date: 03/30/2006
• Status: Expired due to Fees

• Current Assignee: Duke University
• Original Assignee: Duke University
• Inventors: Ramanujam, Nirmala, Wilke, Lee G.
• Primary Examiner(s): NGUYEN, SANG H
• Application Number:
  US11/729,967
• Publication Number:
  US 20090015826A1
• Time in Patent Office: 1,195 Days
• Field of Search: 356/244, 356/246, 356 36- 39, 356/445, 356/344, 422/67, 422/82, 422/86, 382/132, 382/128, 382/140, 436/172, 250/458.1, 250/492.1
• US Class Current: 356/244
• CPC Class Codes: G01N 21/4738 Diffuse reflection precedence is given to G01N21/55 - G01N21/57 if specular component is taken into consideration, e.g. also for testing fluids, fibrous materials

Abstract

The subject matter described herein includes an optical assay system for intraoperative assessment of tumor margins. According to one aspect, the subject matter described herein includes a biological sample containment and illumination apparatus for holding a biological sample for illumination by a plurality of electromagnetic radiation probes. The biological sample containment and illumination apparatus includes a plurality of frame members positioned with respect to each other to form an interior space for receiving a biological sample. At least one of the plurality of frame members includes a plurality of probe receiving locations for receiving a plurality of electromagnetic radiation probes. The probe receiving locations position the probes with respect to the biological sample to allow illumination of plural locations of the biological sample by the probes.

Claims

1. A biological sample containment and illumination apparatus for holding a biological sample for illumination by a plurality of electromagnetic radiation probes, the biological containment and illumination apparatus comprising:

a plurality of frame members positioned with respect to each other to form a single interior volume container for holding a biological sample in an interior region defined by the container,

wherein at least one of the plurality of frame members includes a plurality of probe receiving locations for receiving a plurality of electromagnetic radiation probes and for positioning the probes with respect to the biological sample while the biological sample is located in the interior region defined by the container to allow simultaneous illumination of plural locations of the biological sample by the probes, wherein at least one of the plurality of frame members is translatable with respect to another frame member such that the volume of the interior region can be altered in at least one direction, and wherein the at least one translatable frame member can be translated to vary the volume of the interior region so that the biological sample can be placed proximal to the plurality of probes.

2. The biological sample containment and illumination apparatus of claim 1, wherein at least two of the plurality of frame members are translatable in order to alter the volume of the interior region in at least two directions.

3. The biological sample containment and illumination apparatus of claim 1, wherein the probe receiving locations are adapted to receive fiber optic probes.

4. The biological sample containment and illumination apparatus of claim 3, wherein the probe receiving locations comprise a plurality of apertures for receiving the fiber optic probes.

5. The biological sample containment and illumination apparatus of claim 4, comprising means for controlling advancement of each fiber optic probe in each aperture to position the fiber optic probe adjacent to the biological sample.

6. The biological sample containment and illumination apparatus of claim 5, wherein the means for controlling advancement of each fiber optic probe in each aperture comprises a barrier located at an end of each aperture adjacent to the interior space to prevent the fiber optic probes from entering the interior region.

7. The biological sample containment and illumination apparatus of claim 5, wherein the means for controlling advancement comprises an adapter couplable to one of the fiber optic probes and one of the apertures to limit advancement of the one fiber optic probe in the one aperture.

8. The biological sample containment and illumination apparatus of claim 7, wherein the plurality of fiber optic probes are fixedly attached to each other such that the adapter that limits advancement of the one fiber optic probe in the one aperture limits advancement of the remaining bundled fiber optic probes in their respective apertures.

9. The biological sample containment and illumination apparatus of claim 4, wherein the apertures are spaced from each other so as to avoid cross-talk between adjacent probes.

10. The biological sample containment and illumination apparatus of claim 9, wherein each of the apertures has a diameter of about 3.7 mm and adjacent apertures have a 2 mm radial separation.

11. The biological sample containment and illumination apparatus of claim 3, wherein the fiber optic probes have a sensing depth of between about 1 and 2 mm.

12. The biological sample containment and illumination apparatus of claim 3, wherein the fiber optic probes have a geometry comprising a central core comprising a plurality of illumination fibers surrounded by one or more concentric rings comprising a plurality of collection fibers.

13. The biological sample containment and illumination apparatus of claim 1, wherein the frame members form a parallelepiped structure.

14. The biological sample containment and illumination apparatus of claim 13, wherein the parallelepiped structure is a rectangular parallelepiped.

15. The biological sample containment and illumination apparatus of claim 1, wherein the frame members are translucent or transparent.