Instrument for cassette for sample preparation
First Claim
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1. An instrument, comprising:
- an instrument enclosure configured to receive a plurality of cassettes, each cassette from the plurality of cassettes defining a first chamber and a second chamber, each cassette from the plurality of cassettes including a valve configured to transfer a magnetic particle between the first chamber and the second chamber;
a magnetic particle transfer system disposed within the instrument enclosure, the magnetic particle transfer system including a plurality of valve key shafts and a first actuator assembly, the first actuator assembly configured to move each valve key shaft from the plurality of valve key shafts to actuate the valve of a corresponding cassette from the plurality of cassettes;
a wash buffer pumping system disposed within the instrument enclosure, the wash buffer pumping system including a plurality of engagement plungers and a second actuator assembly, the second actuator assembly configured to move the plurality of engagement plungers between a first position, in which each engagement plunger from the plurality of engagement plungers is engaged with a first pump adjacent a surface of a corresponding cassette from the plurality of cassettes and in communication with the first chamber from the corresponding cassette from the plurality of cassettes, and a second position, in which each engagement plunger from the plurality of engagement plungers is engaged with a second pump adjacent the surface of the corresponding cassette from the plurality of cassettes and in communication with the second chamber from the corresponding cassette from the plurality of cassettes; and
an instrument interface controller configured to control actuation of the magnetic particle transfer system and the wash buffer pumping system.
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Abstract
A parallel processing system for processing samples is described. In one embodiment, the parallel processing system includes an instrument interface parallel controller to control a tray motor driving system, a close-loop heater control and detection system, a magnetic particle transfer system, a reagent release system, a reagent pre-mix pumping system and a wash buffer pumping system.
122 Citations
18 Claims
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1. An instrument, comprising:
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an instrument enclosure configured to receive a plurality of cassettes, each cassette from the plurality of cassettes defining a first chamber and a second chamber, each cassette from the plurality of cassettes including a valve configured to transfer a magnetic particle between the first chamber and the second chamber; a magnetic particle transfer system disposed within the instrument enclosure, the magnetic particle transfer system including a plurality of valve key shafts and a first actuator assembly, the first actuator assembly configured to move each valve key shaft from the plurality of valve key shafts to actuate the valve of a corresponding cassette from the plurality of cassettes; a wash buffer pumping system disposed within the instrument enclosure, the wash buffer pumping system including a plurality of engagement plungers and a second actuator assembly, the second actuator assembly configured to move the plurality of engagement plungers between a first position, in which each engagement plunger from the plurality of engagement plungers is engaged with a first pump adjacent a surface of a corresponding cassette from the plurality of cassettes and in communication with the first chamber from the corresponding cassette from the plurality of cassettes, and a second position, in which each engagement plunger from the plurality of engagement plungers is engaged with a second pump adjacent the surface of the corresponding cassette from the plurality of cassettes and in communication with the second chamber from the corresponding cassette from the plurality of cassettes; and an instrument interface controller configured to control actuation of the magnetic particle transfer system and the wash buffer pumping system. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. An apparatus, comprising:
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an instrument enclosure configured to contain a plurality of cassettes, each cassette from the plurality of cassettes including a valve configured to transfer a particle between a first chamber of the cassette and a second chamber of the cassette while maintaining fluid isolation between the first chamber and the second chamber, each cassette from the plurality of cassettes including a first pump configured to mix a first buffer solution with the particle when the particle is in the first chamber and a second pump configured to mix a second buffer solution with the particle when the particle is in the second chamber, the first pump and the second pump adjacent a surface of the cassette; a particle transfer system disposed within the instrument enclosure, the particle transfer system including a plurality of valve key shafts and a first actuator assembly, the first actuator assembly configured to move the plurality of valve key shafts between a first position and a second position, in the first position each valve key shaft from the plurality of valve key shafts is disposed apart from a corresponding cassette from the plurality of cassettes, in the second position each valve key shaft from the plurality of valve key shafts is engaged with the valve of the corresponding cassette from the plurality of cassettes; and a pumping system disposed within the instrument enclosure, the pumping system including a plurality of engagement plungers and a second actuator assembly, the second actuator assembly configured to move the plurality of engagement plungers between a first engagement plunger position, in which each engagement plunger from the plurality of engagement plungers is engaged with the first pump from the corresponding cassette and a second engagement plunger position in which each engagement plunger from the plurality of engagement plungers is engaged with the second pump from the corresponding cassette. - View Dependent Claims (10, 11, 12, 13, 14, 15)
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16. An apparatus, comprising:
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an instrument enclosure configured to contain a plurality of cassettes, each cassette from the plurality of cassettes defining a first chamber, a second chamber and a third chamber, each cassette from the plurality of cassettes including a first valve configured to transfer a particle between the first chamber and the second chamber while maintaining fluid isolation between the first chamber and the second chamber, and a second valve configured to transfer the particle between the second chamber and the third chamber while maintaining fluid isolation between the second chamber and the third chamber, each cassette from the plurality of cassettes including a first pump configured to mix a first buffer solution with the particle when the particle is in the first chamber and a second pump configured to mix a second buffer with the particle when the particle is in the second chamber; a particle transfer system disposed within the instrument enclosure, the particle transfer system including a plurality of valve key shafts and a first actuator assembly, the actuator assembly configured to move the plurality of valve key shafts between a first position, in which each valve key shaft from the plurality of valve key shafts is engaged with the first valve of a corresponding cassette from the plurality of cassettes, and a second position, in which each valve key shaft from the plurality of valve key shafts is engaged with the second valve of a corresponding cassette from the plurality of cassettes; and a pumping system disposed within the instrument enclosure, the pumping system including a plurality of engagement plunger and a second actuator assembly the second d actuator assembly configured to move the plurality of engagement plungers between a first engagement plunger position, in which each engagement plunger from the plurality of engagement plungers is engaged with the first pump from the corresponding cassette and a second engagement plunger position in which each engagement plunger from the plurality of engagement plungers is engaged with the second pump from the corresponding cassette. - View Dependent Claims (17, 18)
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Specification