Sensor chips

• US 7,759,114 B2
• Filed: 01/06/2006
• Issued: 07/20/2010
• Est. Priority Date: 07/08/2003
• Status: Expired due to Fees

• Current Assignee: Tacnia Pty., Ltd.
• Original Assignee: Tacnia Pty., Ltd.
• Inventors: Johnson, Daniel Luke, Martin, Lisandra Lorraine
• Primary Examiner(s): Haq; Shafiqul
• Application Number:
  US11/327,660
• Publication Number:
  US 20060194343A1
• Time in Patent Office: 1,656 Days
• Field of Search: 436/534, 436/525, 436/532, 436/80, 436/81, 436/823, 530/402, 530/403, 568/21, 568/61, 568/69, 514/836, 424/179.1
• US Class Current: 435/287.2
• CPC Class Codes: B01J 2219/00572 Chemical means
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00653 the compounds being bound to electrodes embedded in or on the solid supports
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/00738 Organic catalysts
• CPC Class Codes: B01J 2219/00745 Inorganic compounds
• CPC Class Codes: B01J 2219/0075 Metal based compounds
• CPC Class Codes: B05D 1/185 applying monomolecular layers B05D1/204 takes precedence
• CPC Class Codes: B05D 2202/40 based on other transition elements
• CPC Class Codes: B05D 3/105 Intermediate treatments
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 40/00 Manufacture or treatment of nanostructures
• CPC Class Codes: C40B 40/10 Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 40/18 Libraries containing only inorganic compounds or inorganic materials
• CPC Class Codes: C40B 70/00 Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
• CPC Class Codes: G01N 33/54353 with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393

Abstract

A method for selectively orienting molecules on a surface of a solid support. The method includes the steps of: (a) attaching a linker molecule to the surface of the solid support, the linker molecule including a head group that is capable of binding to the solid support, and a tail group that is capable of chelating to a metal ion; (b) subsequently treating the solid support with a solution containing the metal ion; (c) attaching a metal ion chelating tag to the molecules to form tagged molecules; and (d) capturing the tagged molecules on the solid support by contacting it with the tagged molecules to form a monolayer of molecules on the surface of the solid support in which a majority of the molecules are held in the same orientation with respect to the surface. The invention also provides a sensor chip formed using the methods of the invention.

Claims

1. A biosensor comprising:

a solid support having a surface;

a plurality of linkers attached to the surface, wherein each linker includes a head group that is bound to the surface, and a multidentate macrocylic tail group comprising 1-acetato-4-benzyl-1,4,7-triazacyclononane chelated to a metal ion; and

a monolayer of molecules immobilised to the solid support by the plurality of linkers and having a binding affinity for an analyte, wherein each molecule of the monolayer contains a metal ion chelating tag that coordinates with the metal ion on a tail group of a linker, thereby holding a majority of the molecules of the monolayer in the same orientation with respect to the surface of the solid support.

2. The biosensor of claim 1 wherein the metal ion is selected from the group consisting of zinc, copper, cobalt, nickel, sodium, potassium, magnesium, calcium, and lithium.

3. The biosensor of claim 1 wherein the tag is a peptide chain containing one or more chelate forming amino acids independently selected from the group consisting of cysteine, lysine, histidine, and arginine.

4. The biosensor of claim 3 wherein the tag is a peptide containing three to six histidine residues.

5. The biosensor of claim 1 wherein the solid support is a noble metal selected from the group consisting of silver, gold, platinum, and palladium.

6. The biosensor of claim 1 wherein the linker includes a spacer between the head and tail groups.

7. The biosensor of claim 6 wherein the spacer is a carbon-based chain between the head and tail groups.

8. The biosensor of claim 7 wherein the carbon-based chain is two to fifteen atoms in length.

9. The biosensor of claim 8 wherein the carbon-based chain is two to six atoms in length.

10. The biosensor of claim 1 wherein the head group is sulfur, oxygen or selenium.

11. The biosensor of claim 1 wherein the monolayer comprises molecules of peptide or protein.

12. The biosensor of claim 11 wherein the tag is covalently bound to the protein at a position on the protein that is separated from the active site or recognition region of the protein.