Real-time linear detection probes: sensitive 5′-minor groove binder-containing probes for amplification (or PCR) analysis
First Claim
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1. A method for distinguishing between wild-type, mutant and heterozygous target polynucleotides, said method comprising:
- (a) contacting a sample containing a target polynucleotide with two probes wherein a first probe is specific for said wild-type target polynucleotide and a second probe is specific for said mutant target polynucleotide, each of said probes having a formula;
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Abstract
Minor groove binder-oligonucleotide probes are provided along with methods for their use wherein the probes have an attached fluorophore which, in an unhybridized form exhibits very low background signal.
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8 Claims
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1. A method for distinguishing between wild-type, mutant and heterozygous target polynucleotides, said method comprising:
(a) contacting a sample containing a target polynucleotide with two probes wherein a first probe is specific for said wild-type target polynucleotide and a second probe is specific for said mutant target polynucleotide, each of said probes having a formula; - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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