Sample analyzer

• US 7,760,340 B2
• Filed: 03/16/2007
• Issued: 07/20/2010
• Est. Priority Date: 03/16/2006
• Status: Active Grant

• Current Assignee: Sysmex Corporation
• Original Assignee: Sysmex Corporation
• Inventors: Kobayashi, Katsushi, Yamamoto, Norimasa, Matsuo, Naohiko, Hoshiko, Susumu
• Primary Examiner(s): Lauchman; L. G
• Application Number:
  US11/724,934
• Publication Number:
  US 20070222973A1
• Time in Patent Office: 1,222 Days
• Field of Search: 356/39, 356/336, 356/338, 600/309, 600/310, 600/320, 600/322, 600/336, 436/49, 436/43, 436/47, 436/48, 436/55, 436/63, 422 63- 65, 422/73
• US Class Current: 356/39
• CPC Class Codes: G01J 2003/123 Indexed discrete filters
• CPC Class Codes: G01N 2021/3148 using three or more wavelengths
• CPC Class Codes: G01N 2021/3155 Measuring in two spectral ranges, e.g. UV and visible
• CPC Class Codes: G01N 2021/3174 Filter wheel
• CPC Class Codes: G01N 2021/825 Agglutination
• CPC Class Codes: G01N 2035/00356 Holding samples at elevated temperature (incubation)
• CPC Class Codes: G01N 2035/0455 Coaxial carousels
• CPC Class Codes: G01N 21/0332 with temperature control control of temperature G05D23/00; cryostats F17C3/08
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/272 for following a reaction, e.g. for determining photometrically a reaction rate (photometric cinetic analysis)
• CPC Class Codes: G01N 21/274 Calibration, base line adjustment, drift correction
• CPC Class Codes: G01N 21/314 with comparison of measurements at specific and non-specific wavelengths dual wavelength spectrometry G01J3/427
• CPC Class Codes: G01N 21/82 producing a precipitate or turbidity
• CPC Class Codes: G01N 2201/0618 Halogene sources
• CPC Class Codes: G01N 2201/0826 Fibre array at source, distributing
• CPC Class Codes: G01N 33/49 Blood chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80
• CPC Class Codes: G01N 35/00603 Reinspection of samples

Abstract

A sample analyzer includes (a) a measuring part for measuring optical information of a sample at first wavelength, second wavelength, and third wavelength, first light of the first wavelength and second light of the second wavelength being absorbed by a second substance but substantially not absorbed by a first substance, and third light of the third wavelength being absorbed by the first substance; and (b) an obtaining part for obtaining content of the first substance in the sample, and content of the second substance in the sample, influence by the second substance being excluded from the content of the first substance, based on the optical information at the first wavelength, second wavelength, and third wavelength measured by the measuring part.

Claims

1. A sample analyzer comprising:

a first measuring part configured for measuring first optical information of a sample at first light of first wavelength, second optical information of a sample at second light of second wavelength, and third optical information of a sample at third light of third wavelength, wherein the first light of the first wavelength and the second light of the second wavelength are absorbed by a first substance but substantially not absorbed by a second substance, and the third light of the third wavelength is absorbed by the second substance;

a preparing part configured for preparing a measurement sample by mixing a reagent with a part of the sample;

a second measuring part configured for optically measuring the measurement sample prepared by the preparing part, wherein the first substance of an excessive content interferes with the measurement by the second measuring part and the second substance of an excessive content interferes with the measurement by the second measuring part; and

a controller configured for executing operations comprising;

determining whether one of content of the first substance and content of the second substance, from which the influence by the first substance is excluded, in the sample is excess or not based on the first, second, and third optical information measured by the first measuring part;

analyzing the measurement sample based on the result of measurement by the second measuring part; and

outputting an analysis result with a flag when one of content of the first and second substances is excess.

2. The sample analyzer according to claim 1, wherein the operations further comprise:

estimating the influence by the first substance on the third optical information based on the first and second optical information; and

correcting the third optical information based on the estimated influence by the first substance on the third optical information;

wherein the determining is carried out based on the first and second optical information and the corrected third optical information.

3. The sample analyzer according to claim 2, wherein the determining is carried out by:

determining whether the content of the first substance is excess or not based on at least one of the first and second optical information; and

determining whether the content of the second substance is excess or not based on the corrected third optical information; and

wherein the outputting comprises;

outputting the analysis result with a first substance flag when content of the first substance is excess; and

outputting the analysis result with a second substance flag when content of the second substance is excess.

4. The sample analyzer according to claim 1, wherein the first light, the second light, and the third light are substantially not absorbed by a third substance, wherein the third substance of an excessive content interferes with the measuring by the second measuring part;

wherein the first measuring part is configured so as to further measure fourth optical information of the sample at fourth light of fourth wavelength, wherein the fourth light of the fourth wavelength is absorbed by the third substance; and

wherein the operations further comprise determining whether content of the third substance, from which influence by the first and second substances is excluded, is excess or not based on the first, second, third, and fourth optical information measured by the first measuring part.

5. The sample analyzer according to claim 4, wherein the operations further comprise:

estimating the influence by the first substance on the third optical information based on the first and second optical information;

correcting the third optical information based on the estimated influence by the first substance on the third optical information;

estimating the influence by the first substance on the fourth optical information based on the first and second optical information;

estimating the influence by the second substance on the fourth optical information based on the corrected third optical information; and

correcting the fourth optical information at the based on the influence by the first substance at the fourth optical information and the influence by the second substance on the fourth optical information; and

wherein the determining is carried out based on corrected fourth optical information.

6. The sample analyzer according to claim 5, wherein the operations further comprise:

obtaining first information relating to presence of the first substance in the sample based on at least one of first optical information and the second optical information;

obtaining second information relating to presence of the second substance in the sample based on the corrected third optical information;

obtaining third information relating to presence of the third substance in the sample based on the corrected fourth optical information; and

outputting at least one of the first, second, and third information.

7. The sample analyzer according to claim 1, wherein the first measuring part comprises:

a light source that emits the first light of the first wavelength, the second light of the second wavelength, and the third light of the third wavelength; and

a light receiver that receives the first light, the second light, and the third light.

8. The sample analyzer according to claim 2, wherein the estimating is carried out by estimating optical information corresponding to the first substance at the third wavelength based on the first optical information and the second optical information;

and

the correcting carried out by correcting the third optical information by eliminating optical information corresponding to the first substance at the third wavelength from the third optical information.

9. The sample analyzer according to claim 8, wherein the optical information is absorbance;

and

the estimating of the influence by the first substance on the third optical information is carried out by estimating absorbance corresponding to the first substance at the third wavelength from a relation derived from the absorbance at the first wavelength and absorbance at the second wavelength.

10. The sample analyzer according to claim 5, wherein the first estimating of the influence by the first substance on the third optical information is carried out by estimating optical information corresponding to the first substance at the third wavelength based on the first and second optical information;

the correcting of the third optical information is carried out by correcting the third optical information by eliminating optical information corresponding to the first substance at the third wavelength from the third optical information

the estimating of the influence by the first substance on the fourth optical information is carried out by estimating optical information corresponding to the first substance at the fourth wavelength based on first optical information and second optical information;

the third estimating of the influence by the second substance on the fourth optical information is carried out by estimating optical information corresponding to the second substance at the fourth wavelength based on the corrected third optical information; and

the correcting of the fourth optical information is carried out by correcting fourth optical information by eliminating optical information corresponding to the first substance at the fourth wavelength, and optical information corresponding to the second substance at the fourth wavelength from the fourth optical information.

11. The sample analyzer according to claim 1, wherein the first wavelength and second wavelength are equal to or greater than 590 nm and equal to or less than 900 nm.

12. The sample analyzer according to claim 1, wherein the third wavelength is equal to or greater than 500 nm and less than 590 nm.

13. The sample analyzer according to claim 4, wherein the fourth wavelength is equal to or greater than 300 nm and less than 500 nm.

14. A sample analyzer comprising:

a first light emitting part configured for emitting light to a blood sample;

a first light receiving part configured for obtaining optical information at a plurality of time points by receiving light of a plurality of wavelengths from the blood sample irradiated by the light;

a sample preparing part configured for preparing a measurement sample by mixing a reagent for measuring coagulation time with a part of the blood sample;

a second light emitting part configured for emitting light to the measurement sample;

a second light receiving part configured for obtaining optical information by receiving light of a plurality of wavelengths over time from the measurement sample; and

a controller configured for executing operations comprising;

measuring, based on the optical information obtained by the first light receiving part, content of an interference substance which interferes with the optical information obtained by the second light receiving part; and

measuring coagulation time of the measurement sample based on the optical information obtained by the second light receiving part.

15. The sample analyzer according to claim 14, wherein the measuring content of the interference substance is carried out by measuring contents of a plurality of interference substances based respectively on the optical information at a plurality of wavelengths obtained by the first light receiving part.

16. The sample analyzer according to claim 14, wherein the first light receiving part is configured so as to obtain first optical information of a sample at light of a first wavelength, second optical information of a sample at light of a second wavelength, and third optical information of a sample at light of a third wavelength;

the measuring content of the interference substance is carried out by measuring at least one of bilirubin, hemoglobin, and chyle in the blood sample based on the first, second, and third optical information obtained by the first light receiving part.

17. The sample analyzer according to claim 16, wherein:

first light of the first wavelength is absorbed by chyle, and is substantially not absorbed by bilirubin and hemoglobin;

second light of the second wavelength is absorbed by chyle and hemoglobin, and is substantially not absorbed by bilirubin;

third light of the third wavelength is absorbed by chyle, hemoglobin and bilirubin;

the measuring content of the interference substance comprises;

estimating influence by chyle on the second and third optical information based on the first optical information; and

estimating influence by hemoglobin on the third optical information based on the second optical information and the influence by chyle on the second optical information;

obtaining contents of bilirubin, hemoglobin, and chyle in the blood sample based on;

the first, second, and third optical information;

the influence by chyle on the second and third optical information; and

the influence by hemoglobin on the third optical information.

18. The sample analyzer according to claim 17, wherein the first light receiving part is configured so as to obtain fourth optical information at fourth light of a fourth wavelength, wherein fourth light of the fourth wavelength being is absorbed by chyle but is substantially not absorbed by bilirubin and hemoglobin;

and

estimating of the influence by chyle on the second and third optical information is carried out based on the first and fourth optical information.

19. The sample analyzer according to claim 14, wherein the first light receiving part is configured so as to obtain first optical information of a sample at first light of a first wavelength, second optical information of a sample at second light of a second wavelength, and third optical information at third light of a third wavelength, wherein the first light of the first wavelength is absorbed by chyle but is substantially not absorbed by bilirubin and hemoglobin, the second light of the second wavelength is absorbed by chyle and hemoglobin but is substantially not absorbed by bilirubin, and the third light of the third wavelength is absorbed by bilirubin, hemoglobin, and chyle;

and

measuring content of the interference substance comprises;

obtaining influence by chyle on the first, second, and third optical information based on first optical information;

obtaining influence by hemoglobin on second optical information and third optical information based on second optical information, and the influence by chyle on second optical information; and

obtaining influence by bilirubin on third optical information based on third optical information, the influence by hemoglobin on third optical information and the influence by chyle on third optical information; and

the sample analyzer further comprises;

an output part for outputting the influence by chyle, the influence by hemoglobin, and the influence by bilirubin on the first, second, and third optical information.

20. The sample analyzer according to claim 14, wherein one of the first light emitting part and the second light emitting part is configured so as to emit light of a plurality of mutually different wavelengths at time divisions.

21. The sample analyzer according to claim 14, wherein the operations further comprise:

deciding whether or not to obtain optical information by the second light receiving part based on measurement result of content of the interference substance.

22. A sample analyzer comprising:

a sample preparing part configured for preparing a measurement sample by mixing a reagent for measuring coagulation time with a blood sample;

a light emitting part configured for emitting light to the prepared measurement sample;

a light receiving part configured for obtaining optical information at a plurality of time points by receiving light of a plurality of wavelengths over time from the measurement sample irradiated by the light; and

a controller configured for executing operations comprising;

correcting optical information obtained by the light receiving part at a time point before the measurement sample indicates coagulation reaction by subtracting a reagent induced absorbance;

measuring content of an interference substance which interferes with optical measurement of the measurement sample based on optical information corrected by the correcting step; and

measuring coagulation time of the measurement sample based on the optical information obtained by the light receiving part.

23. A sample analyzer comprising:

a measuring part configured for measuring first optical information of a sample at first light of first wavelength, second optical information of a sample at second light of second wavelength, and third optical information of a sample at third light of third wavelength, wherein the first light of the first wavelength and the second light of the second wavelength are absorbed by a first substance but substantially not absorbed by a second substance, and wherein the third light of the third wavelength is absorbed by the second substance; and

a controller configured for executing operations comprising;

estimating influence by the first substance on the third optical information based on the first optical information and the second optical information;

correcting the optical information at the third wavelength based on the estimated influence by the first substance on the third optical information;

obtaining content of the first substance in the sample based on the first and second optical information; and

obtaining content of second substance, from which influence by the first substance is excluded, in the sample based on the corrected third optical information.