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Differentiation protocol for making human cardiomyocytes

  • US 7,763,464 B2
  • Filed: 01/21/2005
  • Issued: 07/27/2010
  • Est. Priority Date: 07/12/2001
  • Status: Expired due to Fees
First Claim
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1. A method of differentiating human embryonic stem (hES) cells into cardiomyocyte lineage cells, comprising:

  • a) obtaining hES cells and forming an embryoid body in a cell culture;

    b) culturing the embryoid body from a) in the cell culture with activin, transforming growth factor (TGF), and insulin-like growth factor (IGF),c) subsequently adding bone morphogenic protein (BMP), fibroblast growth factor (FGF), insulin, and platelet-derived growth factor (PDGF);

    to the cell culture from b) and thend) adding a nucleotide analog that affects DNA methylation to the cell culture of c) thereby differentiating hES cells into cardiomyocytes.

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