Reagent container and slide reaction retaining tray, and method of operation

• US 7,767,152 B2
• Filed: 02/03/2006
• Issued: 08/03/2010
• Est. Priority Date: 08/11/2003
• Status: Active Grant

• Current Assignee: Sakura Finetek USA Incorporated (Sakura Finetechnical Co., Ltd.)
• Original Assignee: Sakura Finetek USA Incorporated (Sakura Finetechnical Co., Ltd.)
• Inventors: Stead, Ronald H., Bui, Xuan S.
• Primary Examiner(s): Griffin; Walter D
• Assistant Examiner(s): Mui; Christine T
• Application Number:
  US11/346,876
• Publication Number:
  US 20060171857A1
• Time in Patent Office: 1,642 Days
• Field of Search: 422/102, 422/99, 422/65, 436/46, 359/396
• US Class Current: 422/400
• CPC Class Codes: B01L 2300/041 Connecting closures to device or container
• CPC Class Codes: B01L 2300/0822 Slides
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0457 passive flow or gravitation
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0677 phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 9/52 Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
• CPC Class Codes: G01N 1/312 for samples mounted on planar substrates
• CPC Class Codes: G01N 2035/00138 Slides
• CPC Class Codes: G01N 35/00029 provided with flat sample substrates, e.g. slides G01N35/028 takes precedence
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/112499 with sample on test slide

Abstract

A sample, or slide, retaining tray includes a reagent reservoir, or recess, and a reaction chamber. A slide, or other sample container, can be positioned on the sample retaining tray as desired, with a tissue specimen face down, between a platen on the tray and the slide. A reagent from the reservoir is rendered flowable, such as by heating, and flows from the reservoir into the reaction chamber. Optionally, reagents or other fluids can be provided from above, by dripping onto a drip surface of the sample retaining tray, or below via an inlet port in the platen.

Claims

1. A sample retaining tray comprising:

a reagent holding portion that defines a reagent retaining recess;

a sample holding portion having opposing sidewalls that define a platen that includes a reagent surface that defines an inlet port and an outlet port;

a fluid flow portion that is configured to place the reagent recess in fluid communication with the reagent surface; and

a chamber dimensioned to receive a slide between opposing sidewalls and a bottom surface defining the chamber, wherein a difference between the opposing side walls of the sample holding portion and the opposing sidewalls of the chamber define a reservoir that is in bi-directional fluid communication with the reagent surface.

2. The sample retaining tray of claim 1 further comprising a reagent within the reagent retaining recess.

3. The sample retaining tray of claim 2 wherein the reagent within the reagent retaining recess is contained within a fluidizable matrix.

4. The sample retaining tray of claim 1 wherein the flow portion includes a drip surface that is in fluid communication with the reagent surface that is configured to receive a fluid dispensed from above.

5. The sample retaining tray of claim 4 wherein the drip surface is located between the reagent retaining recess and the platen.

6. The sample retaining tray of claim 1 further comprising at least one slide positioning element configured to position a microscope slide within the sample retaining tray such that the microscope slide is spaced above the reagent surface thereby forming a reaction chamber between the slide and the platen.

7. The sample retaining tray of claim 6 wherein the slide positioning element is configured to hold a slide such that a bottom surface of the slide is angled relative to the reagent surface of the platen.

8. The sample retaining tray of claim 6 further comprising a tissue section positioned within the reaction chamber.

9. The sample retaining tray of claim 1, further comprising a side wall having an angled upper surface and a machine readable identifier positioned on the angled upper surface, the machine readable identifier configured to provide information regarding a reagent contained within the reagent recess.

10. The sample retaining tray of claim 2 further comprising a seal positioned over the reagent recess.

11. The sample retaining tray of claim 1 further comprising a screen on the outlet port.

12. The sample retaining tray of claim 1, wherein the platen includes a shoulder extending upward from the reagent surface of the platen at a distal end of the platen.

13. The sample retaining tray of claim 12 further comprising a rocker positioned adjacent the distal end of the platen, the rocker including a top surface at a lower level relative to the shoulder.

15. The sample retaining tray of claim 1, further comprising:

an identifier positioned on a surface of the sample retaining tray.

16. The sample retaining tray of claim 15, wherein the identifier is a machine readable identifier comprising information regarding a sample held within the sample holding portion of the sample retaining tray.

17. The sample retaining tray of claim 15, wherein the identifier comprises information regarding a reagent contained within the reagent recess, information regarding sample processing or information regarding a sample type.

18. The sample retaining tray of claim 1 further comprising:

a mounting mechanism for releasably securing the sample retaining tray to a mounting surface.

19. The sample retaining tray of claim 18, wherein the mounting mechanism comprises a mounting tab extending from a surface of the tray and an engagement hook dimensioned to be received within a corresponding receiving aperture of the mounting surface.

20. The sample retaining tray of claim 1 wherein the inlet port and the outlet port defined by the reagent surface extend through the reagent surface to an opposite side thereof.

21. The sample retaining tray of claim 1 wherein the opposing sidewalls, the platen and the reagent surface are integrally formed.

14. A sample processing system, comprising:

a plurality of sample retaining trays, wherein each tray includes;

a reagent holding portion that defines a reagent retaining recess;

a sample holding portion having opposing sidewalls that define a platen that includes a reagent surface that defines an inlet port and an outlet port;

a fluid flow portion that is configured to place the reagent recess in fluid communication with the reagent surface; and

a chamber dimensioned to receive a slide between opposing sidewalls and a bottom surface defining the chamber, wherein a difference between the opposing side walls of the sample holding portion and the opposing sidewalls of the chamber define a reservoir that is in bi-directional fluid communication with the reagent surface;

a manifold in fluid communication with the sample retaining trays;

a heating and/or cooling system in thermal communication with the sample retaining trays; and

an external reagent dispensing system in fluid communication with the sample retaining trays.