Method and device for biochemical detection and analysis of subcellular compartments from a single cell
First Claim
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1. A device comprising:
- a substrate to contact a single biological cell;
means to remove a subcellular component of the biological cell;
a chemical reactant selected to facilitate detection of a molecular component present in the subcellular component removed from the biological cell;
a force generator to manipulate the at least one of the biological cell, the molecular component, and the subcellular component;
a separation microchannel on the substrate to separate the molecular component from the subcellular component; and
a controller, the controller comprising a processor and a memory logically coupled to the processor, the memory storing data and machine readable and executable instructions that when executed by the processor, cause a plurality of functions to be carried out, including using the force generator to encapsulate at least one of the subcellular component and the molecular component into an aqueous microdroplet.
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Abstract
A method and system for performing biochemical detection or analysis on micro- and nano-scale subcellular component within a single biological cell is provided. An integrated platform device and method to perform the biochemical analysis is also provided.
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Citations
45 Claims
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1. A device comprising:
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a substrate to contact a single biological cell; means to remove a subcellular component of the biological cell; a chemical reactant selected to facilitate detection of a molecular component present in the subcellular component removed from the biological cell; a force generator to manipulate the at least one of the biological cell, the molecular component, and the subcellular component; a separation microchannel on the substrate to separate the molecular component from the subcellular component; and a controller, the controller comprising a processor and a memory logically coupled to the processor, the memory storing data and machine readable and executable instructions that when executed by the processor, cause a plurality of functions to be carried out, including using the force generator to encapsulate at least one of the subcellular component and the molecular component into an aqueous microdroplet. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 38)
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21. A system comprising:
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a substrate to contact a single biological cell; means to remove a subcellular component of the biological cell; a reactant chamber configured to provide a chemical reactant selected to facilitate the detection of a molecular component present in the subcellular component removed from the biological cell; a force generator to manipulate the at least one of the biological cell, the molecular component, and the subcellular component; a separation microchannel on the substrate to separate the molecular component of the subcellular component; a controller, the controller comprising a processor and a memory logically coupled to the processor, the memory storing data and machine readable and executable instructions that when executed by the processor, cause a plurality of functions to be carried out, including; using a force generator to encapsulate at least one of the subcellular component and the molecular component into an aqueous microdroplet; and reducing a volume of the aqueous microdroplet to increase a relative concentration of the at least one of the subcellular component and the molecular component. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35)
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36. A device comprising:
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a substrate to contact a single biological cell; means to selectively remove a portion of the single biological cell, the portion comprising a subcellular component of the biological cell that is transported across a cellular membrane of the single biological cell, while the single biological cell is disposed in a fluid volume defined by the substrate; means to separate a molecular component from the subcellular component, while the subcellular component is disposed in a fluid volume defined by the substrate, where the molecular component is part of the subcellular component; means to analyze the molecular component; and a controller, the controller comprising a processor and a memory logically coupled to the processor, the memory storing data and machine readable and executable instructions that when executed by the processor, cause a plurality of functions to be carried out, including using a force generator to encapsulate at least one of the subcellular component and the molecular component into an aqueous microdroplet. - View Dependent Claims (37, 39)
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40. A device comprising:
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a substrate configured to contact a single biological cell; a separation microchannel on the substrate to separate the molecular component from the subcellular component; and a controller, the controller comprising a processor and a memory logically coupled to the processor, the memory storing data and machine readable and executable instructions that when executed by the processor, cause a plurality of functions to be carried out, including using a force generator to encapsulate at least one of the subcellular component and the molecular component into an aqueous microdroplet. - View Dependent Claims (41)
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42. A system comprising:
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a substrate configured to contact a single biological cell; a reactant chamber configured to employ a chemical reactant selected to facilitate the detection of a molecular component present in a subcellular component removed from the biological cell; a separation microchannel on the substrate to separate the molecular component from the subcellular component; and a controller configured to implement the functions of; using a force generator to encapsulate at least one of the subcellular component and the molecular component into an aqueous microdroplet; and reducing a volume of the aqueous microdroplet to increase a relative concentration of the at least one of the subcellular component and the molecular component. - View Dependent Claims (43)
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44. A device comprising:
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a substrate to contact a single biological cell; means to remove a subcellular component of the biological cell; means to separate a molecular component from the subcellular component; means to analyze the molecular component; means to encapsulate at least one component selected from a group of components consisting of the subcellular component and the molecular component into an aqueous microdroplet; and means to reduce a volume of the aqueous microdroplet to increase a relative concentration of the at least one of the subcellular component and the molecular component in the aqueous microdroplet.
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45. A device comprising:
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a substrate to contact a single biological cell; means to transport a subcellular component of the biological cell across a cellular membrane of the single biological cell, thus removing the subcellular component from the single biological cell, while the single biological cell is disposed in a fluid volume defined by the substrate; means to separate a molecular component from the subcellular component, while the subcellular component is disposed in a fluid volume defined by the substrate, where the molecular component is part of the subcellular component; means to encapsulate at least one component into an aqueous microdroplet, the at least one component being selected from a group of components consisting of the subcellular component and the molecular component, while the at least one component is disposed in a fluid volume defined by the substrate; means to reduce a volume of the aqueous microdroplet to increase a relative concentration of the at least one of the subcellular component and the molecular component in the aqueous microdroplet; and means to analyze the molecular component.
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Specification