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Method for assessing image focus quality

  • US 7,769,219 B2
  • Filed: 12/11/2006
  • Issued: 08/03/2010
  • Est. Priority Date: 12/11/2006
  • Status: Active Grant
First Claim
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1. A method for determining the quality of focus of a digital image of a biological specimen comprising:

  • (a) obtaining a digital image of the specimen using the specimen imaging apparatus, the digital image comprising a plurality of pixels;

    (b) calculating a measure of small-scale texture in the image, wherein the small-scale texture is measured by squaring the differences of the means of adjacent pairs of pixels across the entire digital image;

    (c) calculating a measure of larger-scale texture in the image, wherein the larger-scale texture is measured by squaring the differences of the means of adjacent triples of pixels across the entire digital image; and

    (d) establishing an estimate of the image focus quality based on a comparison of the small-scale and larger-scale texture measurements.

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