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Methods, kits and compositions for single primer linear isothermal amplification of nucleic acid sequences

  • US 7,771,946 B2
  • Filed: 01/25/2008
  • Issued: 08/10/2010
  • Est. Priority Date: 03/09/2001
  • Status: Expired due to Fees
First Claim
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1. A kit for amplifying a target RNA or the complement of a target RNA comprising a first composite primer, a second composite primer, and a third composite primer, wherein each composite primer comprises an RNA portion and a 3′

  • DNA portion, and wherein the third composite primer is a composite amplification primer and comprises a sequence that is hybridizable to a polynucleotide comprising a complement of the first composite primer.

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