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Methods and devices related to a regulated flow electroporation chamber

  • US 7,771,984 B2
  • Filed: 05/12/2005
  • Issued: 08/10/2010
  • Est. Priority Date: 05/12/2004
  • Status: Active Grant
First Claim
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1. A flow electroporation device comprising:

  • (a) an electroporation chamber having a top and bottom portion containing at least two parallel electrodes, the chamber being formed between the two electrodes and having two chamber ports in the bottom portion of the electroporation chamber and two chamber ports in the top portion of the electroporation chamber;

    (b) at least one sample container that is in fluid communication with the electroporation chamber through a first chamber port in the bottom portion of the chamber and the electroporation chamber is in fluid communication with the sample container through a separate second chamber port in the top portion of the chamber, forming a first fluid path; and

    (c) at least one product container that is in fluid communication with the electroporation chamber through a separate third chamber port in the bottom portion of the chamber and the electroporation chamber is in fluid communication with the product container through a separate fourth chamber port in the top portion of the chamber, forming a second fluid pathwherein at least one fluid path is an aseptic fluid path.

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