Bioluminescence monitor

• US 7,771,990 B2
• Filed: 10/15/2004
• Issued: 08/10/2010
• Est. Priority Date: 10/24/2003
• Status: Active Grant

• Current Assignee: 3M Innovative Properties Company (3M Company)
• Original Assignee: 3M Innovative Properties Company (3M Company)
• Inventors: Jones, Gethin Rhys
• Primary Examiner(s): Griffin; Walter D
• Assistant Examiner(s): EDWARDS, LYDIA E
• Application Number:
  US10/966,203
• Publication Number:
  US 20050089995A1
• Time in Patent Office: 2,125 Days
• Field of Search: 435/288.7, 422/82.05
• US Class Current: 435/288.7
• CPC Class Codes: G01N 21/274 Calibration, base line adjustment, drift correction
• CPC Class Codes: G01N 21/763 Bioluminescence

Abstract

A bioluminescence monitor comprises a photodetector 19 for detecting the amount of light emitted by a sample vessel 11 in a chamber 13. A reflector in the chamber 13 comprises a pair of elongate concave surfaces 16A, 16B which reflect a large proportion of the emitted light towards the photodetector 19. The monitor is calibrated by an LED 20 directed at the photodetector 19 and driven by a pulsed drive signal to provide a light output similar to the light output of an actual sample. The LED 20 is not continuously driven over the calibration period and thus does not heat up significantly enough to effect the level of the light output.

Claims

1. A bioluminescence monitor comprising

a sample chamber for receiving a vessel containing a light-emitting substance,

a photodetector for receiving the emitted light,

an electronic circuit connected to the photodetector, wherein the electronic circuit measures the light received by the photodetector,

calibration means comprising a light emitting diode that directs light at the photodetector and a drive circuit connected to the light emitting diode, wherein the drive circuit applies a pulsed drive signal to the light emitting diode.

2. A bioluminescence monitor as claimed in claim 1, in which the drive circuit drives the light emitting diode with pulses having a period of between 1 and 40 ns.

3. A bioluminescence monitor as claimed in claim 1, in which the drive circuit drives the light emitting diode with successive individual pulses, wherein the period between successive individual pulses is longer than the period of the pulses.

4. A bioluminescence monitor as claimed in claim 1, in which the drive circuit drives the light emitting diode with successive bursts of pulses, wherein the period between successive bursts of pulses is longer than the period of the pulses.

5. A bioluminescence monitor as claimed in claim 1, in which the light emitting diode is mounted in a device which is removably mounted in said chamber.

6. A bioluminescence monitor as claimed in claim 5, in which a closure is provided on said chamber which can be closed once the vessel has been inserted.

7. A bioluminescence monitor as claimed in claim 6, wherein the device is battery powered, and wherein the device comprises a switch for actuating the light emitting diode when the closure is closed.

8. A bioluminescence monitor as claimed in claim 1, in which the light emitting diode is permanently mounted in the chamber.

9. A bioluminescence monitor as claimed in claim 8, in which a reflector is provided in the chamber, the reflector having a front surface arranged to reflect light from the sample towards the photodetector, the light emitting diode being mounted behind the reflector to emit light through the reflector towards the photodetector.

10. A bioluminescence monitor as claimed in claim 9, in which the light emitting diode emits light towards the photodetector through an aperture in the reflector.

11. The bioluminescence monitor of claim 1, further comprising a reflector that reflects emitted light radiating away from the photodetector theretowards.

12. A bioluminescence monitor according to claim 11, wherein the reflector comprises a pair of elongate reflective surfaces extending longitudinally of sample chamber, each surface being concave in section and being directed towards the photodetector.

13. A bioluminescence monitor according to claim 11, in which said light emitting diode emits light towards the photodetector through an aperture in the reflector.

14. A bioluminescence monitor as claimed in claim 11, in which the photodetector is disposed on one side of the sample chamber, on a line which extends radially of the chamber, the reflector being disposed on the opposite side of the chamber with the elongate reflective surfaces being directed along respective lines which converge towards the photodetector.

15. A bioluminescence monitor as claimed in claim 11, in which the photodetector is directed along a line which extends parallel to the longitudinal axis of the sample chamber, a further reflective surface being disposed in front of the photodetector to reflect light emitted radially from the sample and from said reflective surfaces into the photodetector.

16. A bioluminescence monitor as claimed in claim 15, in which the pair of elongate concave reflective surfaces are directed at said further reflective surface.

17. A bioluminescence monitor as claimed in claim 15, in which said further reflective surface lies in the plane which extends at an inclined angle to the directional axis of the photodetector and at an inclined angle to a line which extends radially outwards of the sample vessel at 90 degrees to the longitudinal axis thereof

18. A bioluminescence monitor as claimed in claim 15, in which said further reflective surface is concave.

19. A bioluminescence monitor as claimed in claim 12, in which the pair of elongate concave reflective surfaces are positioned side-by-side and curve in respective opposite directions from a line which extends parallel to the axis of the chamber adjacent said vessel.

20. A bioluminescence monitor as claimed in claim 12, in which the reflective surfaces are provided on a one-piece reflective member positioned inside the chamber.

21. A bioluminescence monitor as claimed in claim 20, in which the one-piece reflective member forms an enclosure having top, bottom and side walls, the reflective surfaces being provided on the side walls of the enclosure.

22. A bioluminescence monitor as claimed in claim 20, in which the internal surfaces of each of the walls of the chamber are reflective.