Directed differentiation of embryonic stem cells into an endoderm cell
First Claim
1. A method of directing differentiation of human embryonic stem cells to an endoderm cell, comprising:
- a. providing chemically dissociated human embryonic stem (hES) cells;
b. aggregating the dissociated hES cells in suspension in order to form embryoid bodies;
c. dissociating the embryoid bodies to provide dissociated single embryonic cells;
d. culturing said dissociated embryonic cells as a monolayer; and
e. exposing said embryonic cells to at least one exogenous factor comprising β
nerve growth factor (NGF) or hepatocyte growth factor (HGF) for an effective period of time to direct differentiation of said dissociated embryonic cells to an embryonic germ layer cell including an endoderm cell.
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Abstract
Methods are described for mapping a pathway of differentiation of a population of embryonic cells that includes exposing the cells to an exogenous factor and measuring gene expression products that are characteristic of a particular cell type or lineage. Directing differentiation of human embryonic cells relies on dissociated embryoid bodies that are then exposed to one or more exogenous factors to enrich a culture for a particular cell type. The differentiated cells may be used for treating a medical condition in a human. Kits for determining differentiation pathways and screening exogenous factors for their utility in differentiation are provided.
12 Citations
3 Claims
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1. A method of directing differentiation of human embryonic stem cells to an endoderm cell, comprising:
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a. providing chemically dissociated human embryonic stem (hES) cells; b. aggregating the dissociated hES cells in suspension in order to form embryoid bodies; c. dissociating the embryoid bodies to provide dissociated single embryonic cells; d. culturing said dissociated embryonic cells as a monolayer; and e. exposing said embryonic cells to at least one exogenous factor comprising β
nerve growth factor (NGF) or hepatocyte growth factor (HGF) for an effective period of time to direct differentiation of said dissociated embryonic cells to an embryonic germ layer cell including an endoderm cell. - View Dependent Claims (2, 3)
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Specification