Laser capture microdissection (LCM) extraction device and device carrier, and method for post-LCM fluid processing

• US 7,776,273 B2
• Filed: 04/26/2001
• Issued: 08/17/2010
• Est. Priority Date: 04/26/2000
• Status: Expired due to Fees

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Head, David F., Richardson, Bruce J., Richardson, Derrick A., Moravick, Keith E., Baer, Thomas M., Reamey, Robert H.
• Primary Examiner(s): Ludlow; Jan M
• Application Number:
  US09/844,187
• Publication Number:
  US 20020001837A1
• Time in Patent Office: 3,400 Days
• Field of Search: 436/177, 422/58, 422/68.1, 422/102
• US Class Current: 422/527
• CPC Class Codes: G01N 1/2813 Producing thin layers of samples on a substrate, e.g. smearing, spinning-on G01N1/30 takes precedence
• CPC Class Codes: G01N 1/30 Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
• CPC Class Codes: G01N 1/40 Concentrating samples
• CPC Class Codes: G01N 2001/284 using local activation of adhesive, i.e. Laser Capture Microdissection
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

The present invention generally discloses an extraction system that provides a locale for fluid processing and extraction on a post-microcapture transfer film. The extraction system includes a transfer film carrier and an extraction device forming a reservoir. The extraction system selectively excludes regions of the transfer film from the reservoir to advantageously reduce contamination due to matter adhered to the transfer film by non-specific transfer.

Claims

1. An extraction unit comprising:

a carrier having an upper portion, a lower portion, and a bottom surface; and

an extraction device for mating with the carrier having a first end, a second end, an inner surface, and an outer surface, comprising;

a shoulder that extends outwardly from the outer surface and disposed between the first and second ends;

a carrier-receiving portion at the first end comprising at least one flange that projects from the shoulder, comprises an inner surface, and is configured to secure the carrier to the extraction device in a compression-fit engagement; and

a conduit interconnected to the carrier-receiving portion;

the conduit extending between a first opening at the carrier-receiving portion and a second opening at the second end;

wherein the carrier mates with the carrier-receiving portion, closes the first opening, seals the first opening to prevent fluid flow through the bottom surface, and forms a reservoir;

wherein the inner surface of the at least one flange contacts the lower portion of the carrier;

wherein the second end is adapted to mate with a vessel such that the vessel is in fluid communication with the conduit; and

wherein specifically transferred material transferred to the carrier by microdissection and non-specifically transferred material are present on the carrier and some non-specifically transferred material is excluded from the reservoir and some specifically transferred material transferred is included in the reservoir.

2. An extraction unit comprising:

a carrier having an upper portion, a lower portion, and a bottom surface; and

a device adapted for mating with the carrier, the device comprising;

a carrier-receiving portion at a first end comprising at least one flange comprising an inner surface and configured to secure the carrier to the device in a compression-fit engagement;

a raised landing portion comprising a landing surface adapted to contact the bottom surface and disposed within the carrier-receiving portion; and

a conduit interconnected to the carrier-receiving portion and extending through the raised landing portion, the conduit having a first opening at the carrier-receiving portion;

wherein the carrier mates with the device at the carrier-receiving portion and contacts the landing surface to form a reservoir with the bottom surface and at least a portion of the conduit, to close the first opening by the bottom surface and to prevent fluid flow through the first opening;

wherein the inner surface of the at least one flange contacts the lower portion of the carrier;

wherein the device covers at least a portion of the bottom surface of the carrier from the reservoir; and

wherein the bottom surface of the carrier has a non-specifically transferred material and specifically transferred material transferred to the carrier by microdissection and the device covers at least a portion of the non-specifically transferred material and at least a portion of the specifically transferred material is included in the reservoir.

3. An extraction unit comprising:

a carrier having an upper portion, a lower portion, and a bottom surface; and

an extraction device for mating with the carrier having a first end, a second end, an inner surface, and an outer surface, comprising;

a shoulder that extends outwardly from the outer surface and disposed between the first and second ends;

a carrier-receiving portion at the first end comprising at least two flanges that each projects from the shoulder, comprises an inner surface, and is configured to secure the carrier to the extraction device in a compression-fit engagement; and

a conduit interconnected to the carrier-receiving portion;

the conduit extending between a first opening at the carrier-receiving portion and a second opening at the second end;

wherein the carrier mates with the carrier-receiving portion, closes the first opening, seals the first opening to prevent fluid flow through the bottom surface, and forms a reservoir;

wherein the inner surface of each of the at least two flanges contacts the lower portion of the carrier; and

wherein the second end is adapted to mate with a vessel such that the vessel is in fluid communication with the conduit.

4. The extraction unit of claim 3, wherein the carrier-receiving portion comprises at least three flanges that each projects from the shoulder, comprises an inner surface, and is configured to secure the carrier to the extraction device in a compression-fit engagement and wherein the inner surface of each of the at least three flanges contacts the lower portion of the carrier.

5. The extraction unit of claim 3, wherein the carrier-receiving portion comprises four flanges that each projects from the shoulder, comprises an inner surface, and is configured to secure the carrier to the extraction device in a compression-fit engagement and wherein the inner surface of each of the at least four flanges contacts the lower portion of the carrier.

6. An extraction unit comprising:

a carrier having an upper portion, a lower portion, and a bottom surface; and

a device adapted for mating with the carrier, the device comprising;

a carrier-receiving portion at a first end comprising at least two flanges that each projects from a shoulder, comprises an inner surface, and is configured to secure the carrier to the extraction device in a compression-fit engagement;

a raised landing portion comprising a landing surface adapted to contact the bottom surface and disposed within the carrier-receiving portion; and

a conduit interconnected to the carrier-receiving portion and extending through the raised landing portion, the conduit having a first opening at the carrier-receiving portion;

wherein the carrier mates with the device at the carrier-receiving portion and contacts the landing surface to form a reservoir with the bottom surface and at least a portion of the conduit, to close the first opening by the bottom surface and to prevent fluid flow through the first opening;

wherein the inner surface of each of the at least two flanges contacts the lower portion of the carrier; and

wherein the device covers at least a portion of the bottom surface of the carrier from the reservoir.

7. The extraction unit of claim 6, wherein the carrier-receiving portion comprises at least three flanges that each projects from the shoulder, comprises an inner surface, and is configured to secure the carrier to the extraction device in a compression-fit engagement and wherein the inner surface of each of the at least three flanges contacts the lower portion of the carrier.

8. The extraction unit of claim 6, wherein the carrier-receiving portion comprises four flanges that each projects from the shoulder, comprises an inner surface, and is configured to secure the carrier to the extraction device in a compression-fit engagement and wherein the inner surface of each of the at least four flanges contacts the lower portion of the carrier.