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Methods and kits using extended rhodamine dyes

  • US 7,776,568 B2
  • Filed: 08/24/2004
  • Issued: 08/17/2010
  • Est. Priority Date: 06/03/1999
  • Status: Expired due to Term
First Claim
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1. A method of PCR enzymatic synthesis comprising amplifying a template DNA with nucleotide triphosphates, a polymerase, and two or more primers wherein the primers are complementary to the template DNA sequence and at least one of the primers is a labelled nucleic acid comprising the formula:


  • NUC-L-DwhereinNUC is a polynucleotide and analogs thereof;

    L is a linkage;

    wherein L is attached to the polynucleotide at a position selected from the 5′

    terminus, the phosphodiester backbone, a nucleobase, and the 3′

    terminus; and

    D is an extended rhodamine dye comprising the structure;

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