Reference microplates and methods for making and using the reference microplates
First Claim
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1. A method for using a reference microplate, said method comprising the steps of:
- placing the reference microplate within an optical interrogation system, wherein said reference microplate includes;
an upper plate having a plurality of through-holes which forms sidewalls for a plurality of wells;
a lower plate, attached to said upper plate, which forms bottom surfaces for said plurality of wells, wherein said lower plate has a biosensor located on said bottom surface of each well and said biosensor has sensing region extending into each well; and
a hardened non-biochemical substance, having a refractive index magnitude within a range of 1.27-1.5, coating said biosensor and at least partially filling one of said wells such that said hardened non-biochemical substance has a depth greater than said sensing region of said biosensor associated with said one of the wells; and
interrogating at least one of the biosensors within the reference microplate.
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Abstract
A reference microplate is described herein which can be used to help calibrate and troubleshoot an optical interrogation system. In one embodiment, the reference microplate has a frame with an array of wells each of which contains an optical biosensor and each optical biosensor is at least partially coated with a substance (e.g., elastomer, optical epoxy). In another embodiment, the reference microplate in addition to having its optical biosensors at least partially covered with a substance (e.g., elastomer, optical epoxy) also has a controllable heating device attached thereto which is used to heat the optical biosensors.
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Citations
17 Claims
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1. A method for using a reference microplate, said method comprising the steps of:
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placing the reference microplate within an optical interrogation system, wherein said reference microplate includes; an upper plate having a plurality of through-holes which forms sidewalls for a plurality of wells; a lower plate, attached to said upper plate, which forms bottom surfaces for said plurality of wells, wherein said lower plate has a biosensor located on said bottom surface of each well and said biosensor has sensing region extending into each well; and a hardened non-biochemical substance, having a refractive index magnitude within a range of 1.27-1.5, coating said biosensor and at least partially filling one of said wells such that said hardened non-biochemical substance has a depth greater than said sensing region of said biosensor associated with said one of the wells; and interrogating at least one of the biosensors within the reference microplate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A method for making a reference microplate, said method comprising the step of:
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placing a non-biochemical substance, having a refractive index magnitude within a range of 1.27-1.5, on top of a biosensor located within a well of a microplate, wherein the biosensor has a sensing region extending into the well, wherein the biosensor is coated with the non-biochemical substance, and wherein the well is at least partially filled with the non-biochemical substance such that the non-biochemical substance has a depth greater than the sensing region of the biosensor; and curing the non-biochemical substance placed on top of the biosensor located within the well of the microplate to form a hardened non-biochemical substance on top of the biosensor located within the well of the microplate. - View Dependent Claims (13, 14, 15, 16, 17)
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Specification