Method for producing unsaturated ω-3-fatty acids in transgenic organisms
CAFCFirst Claim
1. A process for production of compounds comprising one or more C18-, C20-, and/or C22-polyunsaturated fatty acids in a transgenic organism comprising:
- a) introducing, into an organism, at least one nucleic acid sequence which encodes an ω
-3-desaturase that is capable of desaturating C20- or C22-fatty acids, andb) culturing the organism under conditions which permits the production of one or more C18-, C20-, and/or C22-polyunsaturated fatty acids,wherein the ω
-3-desaturase is encoded by a nucleic acid sequence comprising;
i) the nucleic acid sequence of SEQ ID NO;
1,ii) a nucleic acid sequence encoding the amino acid sequence of SEQ ID NO;
2,iii) a nucleic acid sequence having at least 95% identity with the nucleic acid sequence of SEQ ID NO;
1, oriv) a nucleic acid sequence encoding an amino acid sequence having at least 95% identity with the amino acid sequence of SEQ ID NO;
2.
1 Assignment
1 Petition
Reexamination
Accused Products
Abstract
The present invention relates to a process for production of unsaturated ω-3-fatty acids and to a process for production of triglycerides with an elevated content of unsaturated fatty acids, especially of ω-3-fatty acids having more than three double bonds. The invention relates to the production of a transgenic organism, preferably of a transgenic plant or of a transgenic microorganism, with an elevated content of unsaturated ω-3-fatty acids, oils or lipids having ω-3-double bonds as the result of the expression of an ω-3-desaturase from fungi of the family Pythiaceae such as the genus Phytophtora, for example of the genus and species Phytophtora infestans.
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Citations
22 Claims
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1. A process for production of compounds comprising one or more C18-, C20-, and/or C22-polyunsaturated fatty acids in a transgenic organism comprising:
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a) introducing, into an organism, at least one nucleic acid sequence which encodes an ω
-3-desaturase that is capable of desaturating C20- or C22-fatty acids, andb) culturing the organism under conditions which permits the production of one or more C18-, C20-, and/or C22-polyunsaturated fatty acids, wherein the ω
-3-desaturase is encoded by a nucleic acid sequence comprising;i) the nucleic acid sequence of SEQ ID NO;
1,ii) a nucleic acid sequence encoding the amino acid sequence of SEQ ID NO;
2,iii) a nucleic acid sequence having at least 95% identity with the nucleic acid sequence of SEQ ID NO;
1, oriv) a nucleic acid sequence encoding an amino acid sequence having at least 95% identity with the amino acid sequence of SEQ ID NO;
2. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A process for increasing the content of C18-, C20- and/or C22-polyunsaturated fatty acids in oils, lipids, or fatty acids in an organism comprising:
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a) introducing, into an organism, at least one nucleic acid sequence which encodes an ω
-3-desaturase that is capable of desaturating C20- or C22-fatty acids, andb) culturing the organism under conditions which permits the production of C18-, C20- and/or C22-polyunsaturated fatty acids wherein the ω
-3-desaturase is encoded by a nucleic acid sequence comprising;i) the nucleic acid sequence of SEQ ID NO;
1,ii) a nucleic acid sequence encoding the amino acid sequence of SEQ ID NO;
2,iii) a nucleic acid sequence having at least 95% identity with the nucleic acid sequence of SEQ ID NO;
1, oriv) a nucleic acid sequence encoding an amino acid sequence having at least 95% identity with the amino acid sequence of SEQ ID NO;
2. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19)
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20. A process for production of compounds comprising one or more C18-, C20-, and/or C22-polyunsaturated fatty acids in a transgenic organism comprising:
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a) introducing into an organism, at least one nucleic acid sequence which encodes an ω
-3-desaturase that is capable of desaturating C22;
4 ω
-6-fatty acid to C22;
5 ω
-3-fatty acid, andb) culturing the organism under conditions which permits the production of one or more C18-, C20-, and/or C22-polyunsaturated fatty acids. - View Dependent Claims (21, 22)
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Specification