Device and method for multiparametric analysis of microscopic elements

• US 7,777,869 B2
• Filed: 04/14/2006
• Issued: 08/17/2010
• Est. Priority Date: 04/21/2005
• Status: Expired due to Fees

• Current Assignee: Horiba Abx SAS (Horiba Limited)
• Original Assignee: Horiba Abx SAS (Horiba Limited)
• Inventors: Lefevre, Didier, Nerin, Philippe
• Primary Examiner(s): PUNNOOSE, ROY M
• Application Number:
  US11/912,055
• Publication Number:
  US 20080283754A1
• Time in Patent Office: 1,586 Days
• Field of Search: 356/39
• US Class Current: 356/39
• CPC Class Codes: G01N 15/1434 Optical arrangements
• CPC Class Codes: G01N 15/147 the analysis being performed on a sample stream
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 2015/1477 Multiparameters
• CPC Class Codes: G01N 2015/1486 Counting the particles
• CPC Class Codes: G01N 2015/1493 Particle size
• CPC Class Codes: G01N 2021/6419 Excitation at two or more wavelengths
• CPC Class Codes: G01N 2021/6421 Measuring at two or more wavelengths
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53

Abstract

The invention concerns a device (DA) for analyzing microscopic elements, comprising firstly a measuring space (CM) for microscopic elements to be analyzed, secondly at least one source (S) delivering conjugated rays at the measuring space (CM), having at least two different analyzing wavelengths and designed to interact with the microscopic elements in the measuring space (CM) to form interacting rays, thirdly coding means (M) for encoding the rays upstream of the measuring space (CM) with different codes, fourthly optical filtering means (FO) for selectively filtering the interacting rays of fluorescence and/or diffusion depending on their wavelength, fifthly detecting means (DE, DF) for transforming into electric signals part at least of the interacting rays from the measuring space (CM), and sixthly analyzing means (MA) including decoding means (DRE, DRF) for decoding the electric signals to enable data representing the analyzed microscopic elements to be determined.

Claims

1. A device for analyzing microscopic elements, the device comprising:

a measurement space configured to analyze microscopic elements;

at least one source configured to deliver analyzing radiation having at least two different analyzing wavelengths and to interact with said microscopic elements within said measurement space to form interaction radiation;

fluorescent detection means for transforming at least a part of fluorescent interaction radiation originating from said measurement space into electrical signals;

scattering detection means for transforming at least a part of scattering interaction radiation originating from said measurement space into electrical signals;

analysis means for analyzing said electrical signals to allow data representative of said analyzed microscopic elements to be determined, the analysis means including decoding means for decoding said electrical signals to be analyzed as a function of code of origin;

coding means for encoding said analyzing radiation upstream of said measurement space with different codes; and

optical filtering means for selectively filtering any one of fluorescence interaction radiation and scattering interaction radiation as a function of wavelength, upstream of said detection means,

wherein said coded analyzing radiation is conjugated within said measurement space.

2. The device as claimed in claim 1, wherein said at least one source sequentially applies analyzing radiation to said microscopic elements.

3. The device as claimed in claim 1, wherein said at least one source simultaneously applies analyzing radiation to said microscopic elements.

4. The device as claimed in one of claims 1 to 3, wherein said optical filtering means selectively filter said fluorescence interaction radiation whose having wavelengths in fluorescence analysis bands inserted between two analyzing wavelengths and placed beyond a longest analyzing wavelength.

5. The device as claimed in claim 1, wherein said at least one source includes at least two lasers configured to deliver conjugated radiation to said measurement space.

6. The device as claimed in claim 5, wherein said at least two lasers are of the monochrome type.

7. The device as claimed in claim 1, wherein said at least one source includes at least one light-emitting diode.

8. The device as claimed in claim 1, wherein said at least one source includes a source of polychromatic radiation.

9. The device as claimed in claim 8, wherein said source of polychromatic radiation is selected from a group consisting of at least one polychromatic laser with a substantially continuous spectrum, an incandescent lamp and an arc lamp.

10. The device as claimed in claim 1, wherein said at least one source delivers said analyzing radiation according to any one of a continuous mode and a pulsed mode.

11. The device as claimed in claim 1, wherein an intensity of each analyzing radiation beam delivered by said at least one source is adjustable.

12. The device as claimed in one claim 1, wherein said coding means modulate said analyzing radiation in intensity.

13. The device as claimed in claim 1, wherein at least a part of said coding means acts on the radiation downstream of said at least one source to form said analyzing radiation.

14. The device as claimed in claim 1, wherein at least a part of said coding means acts on said at least one source upstream of a distribution output for the analyzing radiation.

15. The device as claimed in claim 14, wherein said at least a part of the coding means is integrated into said at least one source.

16. The device as claimed in claim 1, wherein said coding means include an acoustooptic modulator configured to modulate said radiation delivered by said at least one source to form said analyzing radiation.

17. The device as claimed in claim 1, wherein said fluorescent detection means and said scattering detection means include a multiplicity of photosensitive sensors to detect interaction radiation.

18. The device as claimed in claim 1, wherein said fluorescent detection means and said scattering detection means include a multiplicity of photosensitive sensors to detect interaction radiation, the multiplicity of photosensitive sensors being implanted in a location defined by an axis intercepting a general direction of propagation of the analyzing radiation at an obligatory passage, this axis being oriented at an angle in a range from 0°

to 360°

with respect to the general direction.

19. The device as claimed in claim 1, further including a computer system with a control software interface.

20. A method for analyzing microscopic elements, the method comprising:

illuminating, from at least one source, microscopic elements within a measurement space with analyzing radiation conjugated within said measurement space to form interaction radiation, the analyzing radiation having at least two different analyzing wavelengths and codings according to different chosen codes;

separating and selectively filtering, at an optical filter, any one of fluorescence interaction radiation and scattering interaction radiation as a function of wavelength;

detecting and transforming, at a fluorescent detection unit, filtered fluorescence interaction radiation into electrical signals;

detecting and transforming, at a scattering detection unit, filtered scattering interaction radiation into electrical signals; and

decoding, at a decoder, the electrical signals as a function of code of origin to allow data representative of said analyzed microscopic elements to be determined.

21. The method as claimed in claim 20, wherein said analyzing radiation is applied sequentially to said microscopic elements to be analyzed.

22. The method as claimed in claim 20, wherein said analyzing radiation is applied simultaneously to said microscopic elements.

23. The method as claimed in one of claims 20 to 22, wherein said interaction radiation, whose wavelengths belong to fluorescence analysis bands inserted between two analyzing wavelengths and placed beyond a longest analyzing wavelength, is selectively filtered.

24. The method as claimed in claim 20 wherein a refractive index of one type of microscopic elements analyzed is deduced from signals resulting from transformation of the interaction radiation representative of a scattering process.

25. The method as claimed in claim 20 wherein in a presence of a sample of whole blood, a corpuscular hemoglobin concentration of cells of a erythrocytic strain is determined from said refractive index.

26. The method as claimed in claim 20 wherein a degree of coupling representative of a transfer of energy between fluorochromes is deduced from signals resulting from interaction between said analyzing radiation and two different, spatially close, fluorochromes.

27. A method for analyzing microscopic elements in a field of biological, biochemical, chemical, particulate, morphological analysis, multiparameter flow cytometry, the analysis of charged particles in a liquid or gaseous fluid, or particle size distribution, the method comprising:

illuminating, from at least one source, microscopic elements within a measurement space with analyzing radiation conjugated within said measurement space to form interaction radiation, the analyzing radiation having at least two different analyzing wavelengths and codings according to different chosen codes;

separating and selectively filtering, at an optical filter, any one of fluorescence interaction radiation and scattering interaction radiation as a function of wavelength;

detecting and transforming, at a fluorescent detection unit, filtered fluorescence interaction radiation into electrical signals;

detecting and transforming, at a scattering detection unit, filtered scattering interaction radiation into electrical signals; and

decoding, at a decoder, the electrical signals as a function of code of origin to allow data representative of said analyzed microscopic elements to be determined.

28. A device for analyzing microscopic elements, the device comprising:

a measurement space configured to analyze microscopic elements;

at least one source configured to deliver analyzing radiation having at least two different analyzing wavelengths and to interact with said microscopic elements within said measurement space to form interaction radiation;

a fluorescent detection unit configured to transform at least a part of the interaction radiation originating from said measurement space into electrical signals;

a scattering detection unit configured to transform at least a part of scattering interaction radiation originating from said measurement space into electrical signals;

an analysis unit configured to analyze said electrical signals to allow data representative of said analyzed microscopic elements to be determined, the analysis unit including a decoding unit configured to decode said electrical signals to be analyzed as a function of code of origin;

a coding unit configured to encode said analyzing radiation upstream of said measurement space with different codes; and

optical filtering means configured to selectively filter any one of fluorescence and scattering interaction radiation as a function of wavelength, upstream of said detection means,

wherein said coded analyzing radiation is conjugated within said measurement space.