Detection of analytes in fecal samples

• US 7,781,170 B2
• Filed: 04/25/2003
• Issued: 08/24/2010
• Est. Priority Date: 04/25/2003
• Status: Expired due to Fees

• Current Assignee: IDEXX Laboratories Incorporated
• Original Assignee: IDEXX Laboratories Incorporated
• Inventors: Monn, Michael, Tonelli, Quentin, Groat, Randall
• Primary Examiner(s): Yu; Melanie
• Assistant Examiner(s): Counts; Gary W
• Application Number:
  US10/424,282
• Publication Number:
  US 20040214244A1
• Time in Patent Office: 2,678 Days
• Field of Search: 435/7.1, 435 792- 795, 436/518, 436/535, 436/501, 436/524, 424/151.1
• US Class Current: 435/7.1
• CPC Class Codes: G01N 33/5308 for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites

Abstract

A method for detecting a parasitic worm infection of the digestive tract of a mammal. The method includes detecting the binding of a worm antibody to a worm antigen present in the soluble portion of a fecal sample of infected mammals.

Claims

1. A method for detecting the presence or absence of an intestinal roundworm, hookworm, or whipworm infection in an animal comprising:

contacting a soluble worm component of a fecal sample from the animal with a first antibody specific for a trichloroacetic acid soluble fraction of a disrupted heartworm, wherein the first antibody binds an intestinal roundworm antigen, a hookworm antigen, and a whipworm antigen thereby forming an antigen/antibody complex; and

detecting the presence or absence of the antigen/antibody complex thereby determining the presence or absence of a intestinal roundworm, hookworm, or whipworm infection in the animal.

2. The method of claim 1 wherein the step of detecting further comprises providing a second antibody that binds to the antigen of the antigen/antibody complex, wherein the second antibody is specific for a worm antigen, wherein the worm antigen is selected from the group consisting of intestinal roundworm antigen, hookworm antigen, and a whipworm antigen.

3. The method of claim 1 wherein the animal is canine or feline.

4. The method of claim 2, wherein the first or second antibody is attached to a phase that is immiscible with the sample.

5. The method of claim 4, wherein the phase is a solid phase.

6. The method of claim 2 wherein the first or second antibody is bound to a label.

7. The method of claim 6 wherein the label is an enzyme, a colloidal particle, a radionuclide or a fluorophore.

8. A method for detecting the presence or absence of a parasitic worm antigen in animal feces comprising:

(a) forming a mixture comprising a soluble portion of a fecal sample and an antibody specific for a trichloroacetic acid soluble fraction of a disrupted, heartworm wherein the antibody binds an intestinal roundworm antigen, a hookworm antigen, and a whipworm antigen;

(b) detecting whether the antibody binds an antigen in the fecal sample, wherein the antigen is selected from the group consisting of intestinal roundworm antigen, hookworm antigen, and whipworm antigen, thereby determining the presence or absence of a parasitic worm antigen in feces.

9. The method of claim 8, wherein the antibody is attached to a phase that is immiscible with the sample.

10. The method of claim 9, wherein the phase is a solid phase.

11. The method of claim 8 wherein the antibody is bound to a detectable moiety.

12. The method of claim 11, wherein the detectable moiety is an enzyme, a colloidal particle, a radionuclide or a fluorophore.

13. A method for detecting whether the digestive tract of an animal is infected with tapeworms, intestinal roundworms, hookworms, or whipworms comprising:

immobilizing antibody specific for a trichloroacetic acid soluble fraction of a disrupted heartworm on a solid phase, wherein the antibody binds a tapeworm antigen, an intestinal roundworm antigen, a hookworm antigen and a whipworm antigen;

adding a soluble portion of a fecal sample from the animal to the solid phase;

detecting the binding of a parasitic worm antigen to the immobilized antibody, wherein the parasitic worm antigen is one or more of a tapeworm antigen, an intestinal roundworm antigen, a hookworm antigen, or a whipworm antigen, thereby detecting whether the animal is infected with tapeworms, intestinal roundworms, hookworms, or whipworms.

14. The method of claim 13 wherein detecting the binding of the parasitic worm antigen to the immobilized antibody comprises contacting the solid phase with a worm antibody conjugated to a label.

15. The method of claim 13 further comprising adding a worm antigen conjugated to a label to the soluble portion of a fecal sample from the animal prior to addition to the solid phase and determining the presence, absence or amount of the binding of the labeled antigen to the antibody.

16. The method of claim 13, further comprising testing a blood sample from the mammal to detect the presence or absence of heartworm antigen in the blood sample.