Screening methods using normal human liver cell line

• US 7,781,186 B2
• Filed: 01/19/2006
• Issued: 08/24/2010
• Est. Priority Date: 09/25/2000
• Status: Expired due to Fees

• Current Assignee: United States Army (U.S. Department Of Defense)
• Original Assignee: The United States Of America As Represented By The Secretary Of The Army
• Inventors: Prachumsri, Jetsumon, Yimamnuaychok, Nongnuch
• Primary Examiner(s): MINNIFIELD, NITA M
• Application Number:
  US11/335,352
• Publication Number:
  US 20060160216A1
• Time in Patent Office: 1,678 Days
• Field of Search: None
• US Class Current: 435/32
• CPC Class Codes: C12N 2500/05 Inorganic components
• CPC Class Codes: C12N 2500/30 Organic components metal chelators C12N2500/10; calcitonin C12N2500/14; transferrin C12N2500/24
• CPC Class Codes: C12N 2500/36 Lipids
• CPC Class Codes: C12N 2500/38 Vitamins
• CPC Class Codes: C12N 2500/62 DMSO
• CPC Class Codes: C12N 2501/30 Hormones derived from pro-opiomelanocortin, pro-enkephalin or pro-dynorphin C12N2501/85
• CPC Class Codes: C12N 2501/335 Glucagon; Glucagon-like peptide [GLP]; Exendin
• CPC Class Codes: C12N 2501/375 Thyroid stimulating hormone [TSH]
• CPC Class Codes: C12N 2501/39 Steroid hormones
• CPC Class Codes: C12N 2503/00 Use of cells in diagnostics
• CPC Class Codes: C12N 2503/02 Drug screening
• CPC Class Codes: C12N 2510/02 Cells for production
• CPC Class Codes: C12N 2510/04 Immortalised cells
• CPC Class Codes: C12N 5/067 Hepatocytes
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

In this application is described the establishment and maintenance of a normal human hepatocyte cell line able to support complete development of malaria parasite development in vitro. The cell line can be used in methods for screening compounds affecting normal human liver cells or normal human cells infected with malaria parasites, measuring uptake of drugs or chemicals into normal human liver cells, and measuring metabolism of a drug in a normal human liver cell. Other advantages and uses of the cell line are also described.

Claims

1. A method for screening a compound affecting malaria parasite development in a human liver cell infected with said parasite comprising

incubating components comprising the compound and at least one immortalized normal human hepatocyte which has been infected with the parasite under conditions sufficient to allow the compound and cell to interact;

and

determining the effect of the compound on the parasite.

2. The method of claim 1 wherein said immortalized normal human hepatocyte is from HC-04.

3. The method of claim 2 wherein said malaria parasite is P. falciparum or P. vivax.

4. A method for screening a compound affecting a normal human liver cell infected with a malaria parasite:

incubating components comprising the compound and at least one immortalized normal human hepatocyte infected with the parasite under conditions sufficient to allow the compound and cell to interact; and

determining the effect of the compound on the cell.

5. The method of claim 4 wherein said immortalized normal human hepatocyte is from HC-04.

6. The method of claim 5 wherein said malaria parasite is P. falciparum or P. vivax.