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Solventless extraction process

  • US 7,781,193 B2
  • Filed: 07/24/2007
  • Issued: 08/24/2010
  • Est. Priority Date: 01/19/2000
  • Status: Expired due to Fees
First Claim
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1. A process for obtaining lipids from microorganisms comprising:

  • (a) releasing intracellular lipids of cells of the microorganisms grown in a fermentation medium into the fermentation medium by lysing the cells, rupturing the cells, or permeabilizing the cells;

    (b) producing a phase separated mixture from the fermentation medium comprising a heavy layer and a light layer, wherein said heavy layer comprises an aqueous solution and said light layer comprises said lipids;

    (c) separating said heavy layer from said light layer; and

    (d) obtaining said lipids from said light layer.

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