Particle on membrane assay system
First Claim
1. A method of detecting at least one analyte in a fluid comprising:
- forming a mixture of one or more first particles with the fluid, wherein each particle comprises a receptor configured to interact with at least a first analyte, and wherein particles that interact with at least a first analyte undergo a detectable spectroscopic change;
passing the mixture of first particles and fluid across a porous membrane and a particle-based sensor array disposed in an analyte detection device, wherein the analyte detection device is configured to capture the first particles on the porous membrane allowing at least a second analyte to flow through the membrane and contact a second particle, wherein the analyte detection device comprises;
a first sensor comprising the porous membrane configured to capture the first particles, a top member positioned at a spaced distance above the porous membrane such that a first cavity is formed between the top member and the porous membrane, wherein the top member covers at least a portion of the porous membrane, and wherein the top member is substantially transparent to light, and a bottom member positioned below the porous membrane, wherein the bottom member is configured to receive fluid flowing through the porous membrane, anda second sensor in series comprising the particle-based sensor array, wherein the particle-based sensor array is integral with the porous membrane and comprises a plurality of wells fabricated in an ordered array, wherein at least the second particle is positioned within a well and is configured to interact with the second analyte during use to produce a spectroscopic signal;
detecting spectroscopic signals produced by the first particles captured on the porous membrane and the second particles in the particle-based sensor array; and
determining if one or more analyte is present based on the detected spectroscopic signals.
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Accused Products
Abstract
Described herein is an analyte detection device and method related to a portable instrument suitable for point-of-care analyses. In some embodiments, a portable instrument may include a disposable cartridge, an optical detector, a sample collection device and/or sample reservoir, reagent delivery systems, fluid delivery systems, one or more channels, and/or waste reservoirs. Use of a portable instrument may reduce the hazard to an operator by reducing an operator'"'"'s contact with a sample for analysis. The device is capable of obtaining diagnostic information using cellular- and/or particle-based analyses and may be used in conjunction with membrane- and/or particle-based analysis cartridges. Analytes, including proteins and cells and/or microbes may be detected using the membrane and/or particle based analysis system.
139 Citations
15 Claims
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1. A method of detecting at least one analyte in a fluid comprising:
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forming a mixture of one or more first particles with the fluid, wherein each particle comprises a receptor configured to interact with at least a first analyte, and wherein particles that interact with at least a first analyte undergo a detectable spectroscopic change; passing the mixture of first particles and fluid across a porous membrane and a particle-based sensor array disposed in an analyte detection device, wherein the analyte detection device is configured to capture the first particles on the porous membrane allowing at least a second analyte to flow through the membrane and contact a second particle, wherein the analyte detection device comprises; a first sensor comprising the porous membrane configured to capture the first particles, a top member positioned at a spaced distance above the porous membrane such that a first cavity is formed between the top member and the porous membrane, wherein the top member covers at least a portion of the porous membrane, and wherein the top member is substantially transparent to light, and a bottom member positioned below the porous membrane, wherein the bottom member is configured to receive fluid flowing through the porous membrane, and a second sensor in series comprising the particle-based sensor array, wherein the particle-based sensor array is integral with the porous membrane and comprises a plurality of wells fabricated in an ordered array, wherein at least the second particle is positioned within a well and is configured to interact with the second analyte during use to produce a spectroscopic signal; detecting spectroscopic signals produced by the first particles captured on the porous membrane and the second particles in the particle-based sensor array; and determining if one or more analyte is present based on the detected spectroscopic signals. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification