Chimeric immunomodulatory compounds and methods of using the same—III

• US 7,785,610 B2
• Filed: 12/23/2002
• Issued: 08/31/2010
• Est. Priority Date: 06/21/2001
• Status: Active Grant

• Current Assignee: Dynamax Technologies Limited
• Original Assignee: Dynamax Technologies Limited
• Inventors: Fearon, Karen L., Dina, Dino, Tuck, Stephen F.
• Primary Examiner(s): Le; Emily M.
• Application Number:
  US10/328,578
• Publication Number:
  US 20030225016A1
• Time in Patent Office: 2,808 Days
• Field of Search: None
• US Class Current: 424/278.1
• CPC Class Codes: C07H 15/18 Acyclic radicals, substituted by carbocyclic rings
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Abstract

The invention provides immunomodulatory compounds and methods for immunomodulation of individuals using the immunomodulatory compounds.

Claims

1. A method of modulating an immune response in an individual comprising administering to an individual a branched chimeric immunomodulatory compound (CIC) in an amount sufficient to modulate an immune response in said individual, wherein:

the CIC comprises at least three nucleic acid moieties and one or more non-nucleic acid, spacer moieties;

each nucleic acid moiety is covalently joined to at least one non-nucleic acid spacer moiety;

at least one nucleic acid moiety comprises the sequence 5′

-CG-3′

; and

said CIC has immunomodulatory activity.

2. The method of modulating an immune response of claim 1 wherein the CIC has a fork structure, an H structure, a comb structure, or a central spacer structure.

5. A method of claim 1 wherein the immunomodulatory activity is an increase in interferon-α

.

6. A method of claim 1 wherein at least one of the nucleic acid moieties comprises the sequence TCG in the 5′

position.

7. A method of claim 1, wherein two or more of the nucleic acid moieties comprises the sequence TCG in the 5′

position.

3. A method of modulating an immune response of a population of cells, comprising contacting the population with a composition comprising a branched chimeric immunomodulatory compound (CIC) wherein:

the CIC comprises at least three nucleic acid moieties and one or more non-nucleic acid spacer moieties;

each nucleic acid moiety is covalently joined to at least one non-nucleic acid spacer moiety;

at least one nucleic acid moiety comprises the sequence 5′

-CG-3′

; and

said CIC has immunomodulatory activity.

4. The method of modulating an immune response of claim 3, wherein the CIC has a fork structure, an H structure, a comb structure, or a central spacer structure.