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Detecting methylated mammalian nucleic acid in stool

  • US 7,785,772 B2
  • Filed: 01/23/2008
  • Issued: 08/31/2010
  • Est. Priority Date: 01/23/2007
  • Status: Expired due to Fees
First Claim
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1. A method for enriching methylated mammalian nucleic acid if present in a stool sample, wherein said method comprises:

  • (a) contacting nucleic acid from a stool sample with an MBD polypeptide to form a nucleic acid-MBD polypeptide complex;

    (b) eluting bacterial nucleic acid from said MBD polypeptide of the formed nucleic acid-MBD polypeptide complex using a buffer with less than or equal to 0.6 M NaCl, and(c) eluting methylated mammalian nucleic acid from said complex if said stool sample contained methylated mammalian nucleic acid using a buffer with greater than 0.6 M NaCl, thereby forming an enriched, methylated mammalian nucleic acid sample.

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