Compositions and methods related to flavivirus envelope protein domain III antigens
First Claim
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1. A method of screening for West Nile Virus in a subject or animal host comprising:
- a) contacting a sample from the subject or animal with a composition comprising a flavivirus envelope protein domain III polypeptide under conditions that permit formation of specific immunocomplex between an antibody in the sample and the envelope protein domain III peptide, wherein the envelope protein domain III peptide is a West Nile virus envelope protein domain III peptide and has a length of 103 to 118 amino acids and comprises the amino acid sequence of SEQ ID NO;
11; and
b) detecting whether a specific immunocomplex is formed.
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Abstract
The present invention concerns methods and compositions involving flavivirus envelope protein domain III antigens for the detection of virus and detection of antibodies against the virus. Such methods and compositions may be used to detect TBE serocomplex viruses or West Nile virus infection in a subject, patient, animal or biological fluid. The present invention also concerns kits for implementing such methods. In some embodiments, kits contain a recombinant TBE serocomplex virus or West Nile virus envelope protein domain III antigen.
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13 Claims
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1. A method of screening for West Nile Virus in a subject or animal host comprising:
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a) contacting a sample from the subject or animal with a composition comprising a flavivirus envelope protein domain III polypeptide under conditions that permit formation of specific immunocomplex between an antibody in the sample and the envelope protein domain III peptide, wherein the envelope protein domain III peptide is a West Nile virus envelope protein domain III peptide and has a length of 103 to 118 amino acids and comprises the amino acid sequence of SEQ ID NO;
11; andb) detecting whether a specific immunocomplex is formed. - View Dependent Claims (2, 3, 4, 5)
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- 6. A composition comprising an isolated West Nile virus envelope protein domain III peptide, wherein the peptide has a length of 103 to 118 amino acids and comprises the amino acid sequence of SEQ ID NO:
- 9. A kit for screening for West Nile virus antibodies, in a suitable container, comprising at least one envelope protein domain III polypeptide, wherein the at least one envelope protein domain III polypeptide is a West Nile virus envelope protein domain III peptide, wherein the peptide has a length of 103 to 118 amino acids and comprises the amino acid sequence of SEQ ID NO:
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10. A kit for screening for West Nile virus antibodies in a subject comprising:
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a) an assay plate comprising a multiplicity of microtiter wells comprising a composition comprising an envelope protein domain III polypeptide capable of binding a West Nile virus antibody in the sample that can specifically bind to the envelope protein domain III polypeptide wherein at least one domain III polypeptide is a West Nile virus envelope protein domain III peptide, wherein the peptide has a length of 103 to 118 amino acids and comprises the amino acid sequence of SEQ ID NO;
11; andb) a container comprising a labeled secondary antibody having specific binding affinity for a West Nile virus antibody in the sample that can specifically bind to the envelope protein domain III polypeptide.
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Specification