Apparatus to automatically lyse a sample

• US 7,785,868 B2
• Filed: 11/29/2005
• Issued: 08/31/2010
• Est. Priority Date: 12/02/2004
• Status: Expired due to Fees

• Current Assignee: MicroFluidic Systems, Inc.
• Original Assignee: MicroFluidic Systems, Inc.
• Inventors: Pourahmadi, Farzad, Yuan, Bob, Northrup, Allen
• Primary Examiner(s): Beisner; William H
• Application Number:
  US11/290,653
• Publication Number:
  US 20060121603A1
• Time in Patent Office: 1,736 Days
• Field of Search: 435/259, 435/286.7, 435/303.3, 435/306.1, 241/2, 366/114, 366/127, 134/107, 134/901, 310/325
• US Class Current: 435/306.1
• CPC Class Codes: B01F 31/86 with vibration of the receptacle or part of it
• CPC Class Codes: C12M 47/06 Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material lysis of microorganisms C12N1/06; extracting or separating nucleic acids from biological samples C12N15/1003

Abstract

A standalone bench top laboratory instrument designed to disrupt, or lyse, cells, spores and tissue samples using ultrasonic energy. The lysing device is programmable, allowing the user control over the sample volume, sonication power level, and lysing duration in order to optimize lysing protocols for specific targets. Once a lysing protocol is entered, the device automatically lyses the sample according to the entered lysing protocol. The lysing device also provides a cooling feature, enabled by a heat exchanging sub-assembly, to prevent the sample from exceeding a maximum set temperature.

Claims

1. An apparatus to control temperature while lysing a sample using ultrasonic energy, the apparatus comprising:

a. a sample container to hold the sample to be lysed;

b. an ultrasonic generator in contact with the sample container to provide ultrasonic energy to the sample;

c. a heat exchanging sub-assembly coupled to the sample container, wherein the heat exchanging sub-assembly includes at least one spring-loaded heat block that is pressed into contact with the sample container, a heat sink, and a thermoelectric cooler thermally coupled between the at least one heat block and the heat sink, further wherein the heat exchanging sub-assembly is configured such that heat generated within the sample container during application of the ultrasonic energy is transferred from the sample container to the heat exchanging sub-assembly.

2. The apparatus of claim 1 wherein the ultrasonic generator comprises a sonication horn.

3. The apparatus of claim 2 wherein the sonication horn includes a transducer tip, further wherein the transducer tip is positioned to contact a first surface of the sample container.

4. The apparatus of claim 3 further comprising a compression spring coupled to the sonication horn, wherein the compression spring is calibrated such that a predetermined force is maintained by the transducer tip against the first surface of the sample container.

5. The apparatus of claim 3 wherein the sample container is sealed and the transducer tip contacts an external surface of the sample container, thereby providing a sealed environment within the sample container.

6. The apparatus of claim 1 wherein the ultrasonic energy lyses a cell, a spore, or a tissue within the sample.

7. The apparatus of claim 1 further comprising a variable programming circuit coupled to the ultrasonic generator to control a power and a duration of the ultrasonic energy applied.

8. The apparatus of claim 7 wherein the apparatus is partitioned into a lysing engine compartment and an electronics compartment that are thermally isolated from each other, wherein the sample container, the ultrasonic generator, and the heat exchanging sub-assembly are positioned in the lysing engine compartment, and the variable programming circuit is positioned in the electronics compartment such that the variable programming circuit is thermally isolated from the sample container, the ultrasonic generator, and the heat exchanging sub-assembly.

9. The apparatus of claim 7 further comprising a temperature sensor coupled to the heat exchanging sub-assembly.

10. The apparatus of claim 9 wherein the variable programming circuit is coupled to the temperature sensor to receive temperature information, further wherein the variable programming circuit provides control signals to the ultrasonic generator in response to the temperature information.

11. The apparatus of claim 7 further comprising a user interface coupled to the variable programming circuit, the user interface is used to enter operation parameters.

12. The apparatus of claim 11 wherein the operation parameters include a volume size of the sample, a power level of the ultrasonic energy to be applied, and a duration for which the ultrasonic energy is to be applied.

13. The apparatus of claim 1 wherein the sample container is removable from the apparatus.

14. An apparatus to lyse a sample using ultrasonic energy, the apparatus comprising:

a. a sample container to hold the sample to be lysed;

b. an ultrasonic generator in contact with the sample container to provide ultrasonic energy to the sample;

c. a heat exchanging sub-assembly coupled to the sample container to facilitate heat transfer to and from the sample container, wherein the heat exchanging sub-assembly includes at least one spring-loaded heat block that is pressed into contact with the sample container;

d. a variable programming circuit to provide control signals to the ultrasonic generator according to a provided lysing protocol.

15. The apparatus of claim 14 wherein the ultrasonic generator comprises a sonication horn.

16. The apparatus of claim 15 wherein the sonication horn includes a transducer tip, further wherein the transducer tip is positioned to contact a first surface of the sample container.

17. The apparatus of claim 16 further comprising a compression spring coupled to the sonication horn, wherein the compression spring is calibrated such that a predetermined force is maintained by the transducer tip against the first surface of the sample container.

18. The apparatus of claim 16 wherein the sample container is sealed and the transducer tip contacts an external surface of the sample container, thereby providing a sealed environment within the sample container.

19. The apparatus of claim 14 wherein the heat exchanging sub-assembly further comprises a thermoelectric cooler coupled to the at least one heat block.

20. The apparatus of claim 14 wherein the ultrasonic energy lyses a cell, a spore, or a tissue within the sample.

21. The apparatus of claim 14 wherein the lysing protocol specifies a power level of the ultrasonic energy, a duration for which the ultrasonic energy is to be applied, or a volume size of the sample included within the sample container.

22. The apparatus of claim 21 further comprising a user interface coupled to the variable programming circuit, the user interface is used to enter the lysing protocol.

23. The apparatus of claim 14 further comprising a temperature sensor coupled to the heat exchanging sub-assembly.

24. The apparatus of claim 23 wherein the variable programming circuit is coupled to the temperature sensor to receive temperature information, further wherein the variable programming circuit provides control signals to the ultrasonic generator in response to the temperature information.

25. An apparatus to lyse a sample using ultrasonic energy, the apparatus comprising:

a. a sample stored within a sealed environment;

b. a sonication horn positioned to contact an external surface of the sealed environment, wherein the sonication horn generates ultrasonic energy which is provided to the sample via the external surface; and

c. a heat exchanging sub-assembly coupled to the sealed environment to facilitate heat transfer to and from the sealed environment, wherein the heat exchanging sub-assembly includes at least one spring-loaded heat block that is pressed into contact with the sealed environment.

26. The apparatus of claim 25 wherein the sonication horn includes a transducer tip, further wherein the transducer tip is positioned to contact the external surface of the sealed environment.

27. The apparatus of claim 26 further comprising a compression spring coupled to the sonication horn, wherein the compression spring is calibrated such that a predetermined force is maintained by the transducer tip against the external surface of the sealed environment.

28. The apparatus of claim 25 wherein the sealed environment comprises a vial with a cap.

29. The apparatus of claim 25 wherein the heat exchanging sub-assembly further comprises a thermoelectric cooler coupled to the at least one heat block.

30. The apparatus of claim 25 wherein the ultrasonic energy lyses a cell, a spore, or a tissue within the sample.

31. The apparatus of claim 25 further comprising a variable programming circuit coupled to the ultrasonic generator to control a power and a duration of the ultrasonic energy applied.

32. The apparatus of claim 31 further comprising a temperature sensor coupled to the heat exchanging sub-assembly.

33. The apparatus of claim 32 wherein the variable programming circuit is coupled to the temperature sensor to receive temperature information, further wherein the variable programming circuit provides control signals to the ultrasonic generator in response to the temperature information.

34. The apparatus of claim 31 further comprising a user interface coupled to the variable programming circuit, the user interface is used to enter operation parameters.

35. The apparatus of claim 34 wherein the operation parameters include a volume size of the sample, a power level of the ultrasonic energy to be applied, and a duration for which the ultrasonic energy is to be applied.