×

Sonication to selectively lyse different cell types

  • US 7,785,869 B2
  • Filed: 02/09/2006
  • Issued: 08/31/2010
  • Est. Priority Date: 09/19/2003
  • Status: Expired due to Fees
First Claim
Patent Images

1. An apparatus to selectively lyse a specific type of cell, the apparatus comprising:

  • a. a sonication chamber to receive a sample and to provide a first sonication energy, the sample having at least two different cell types, wherein a first cell type is lysed at the first sonication energy, further wherein the sonication chamber is configured to selectively lyse the first cell type, thereby forming a first lysate, while leaving one or more non-lysed cell types within a remaining sample;

    b. separating means to separate the first lysate from the sample;

    c. a collection vessel to collect the first lysate; and

    d. microfluidic circuitry to couple the sonication chamber, the separating means, and the collection vessel, wherein the microfluidic circuitry is configured to enable back-flow capability for moving the remaining sample including the one or more non-lysed cell types from the separating means to the sonication chamber, further wherein the sonication chamber is configured to lyse a second cell type within the remaining sample.

View all claims
  • 3 Assignments
Timeline View
Assignment View
    ×
    ×