Method and apparatus for measurement of optical properties in tissue

• US 7,787,129 B2
• Filed: 01/31/2007
• Issued: 08/31/2010
• Est. Priority Date: 01/31/2006
• Status: Active Grant

• Current Assignee: Board of Trustees of The University of Illinois
• Original Assignee: Board of Trustees of The University of Illinois
• Inventors: Zysk, Adam M., Leigh, Matthew S., Adie, Steven G., Sampson, David D., Armstrong, Julian J., Boppart, Stephen A.
• Primary Examiner(s): Chowdhury; Tarifur
• Assistant Examiner(s): Hansen; Jonathan M
• Application Number:
  US11/669,561
• Publication Number:
  US 20070203404A1
• Time in Patent Office: 1,308 Days
• Field of Search: 356/477, 356/479, 356/481, 356/482, 356/517, 356/498, 356/454, D24/138
• US Class Current: 356/481
• CPC Class Codes: A61B 10/0233 Pointed or sharp biopsy instruments
• CPC Class Codes: A61B 5/0066 Optical coherence imaging
• CPC Class Codes: A61B 5/0084 for introduction into the body, e.g. by catheters A61B5/1459 takes precedence
• CPC Class Codes: A61B 5/0091 for mammography
• CPC Class Codes: A61B 5/6848 Needles

Abstract

A method of analyzing tissue includes inserting a radiation source into tissue, impinging radiation upon the tissue, obtaining a sample signal of the radiation that impinges upon the tissue, and determining a refractive index of the tissue from the sample signal. The method may also include determining at least one other optical property of the tissue. The method may provide for identifying tissue as part of a biopsy method. A device for analyzing tissue may include a low-coherence interferometer and a probe optically coupled to the interferometer, where the probe includes a radiation source.

Claims

1. A method of analyzing tissue, comprising:

inserting a probe into tissue;

impinging radiation upon the tissue;

obtaining a sample signal of the radiation from the tissue; and

determining a refractive index of the tissue from the sample signal;

wherein the probe comprises;

a housing,

a piercing tip, at a distal end of the housing,

a groove, in the housing,

a radiation source, and

a radiation collector,

wherein an optical path extends across the groove between the radiation source and the radiation collector.

2. The method of claim 1, further comprising determining at least one other optical property of the tissue.

3. The method of claim 2, where the at least one other optical property is selected from the group consisting of the attenuation coefficient, the scattering profile, the anisotropy factor, the birefringence, the spectral shift, and the texture.

4. The method of claim 1, where the impinging radiation comprises passing low-coherence radiation through an optical fiber to the tissue;

and the determining comprises combining the sample signal with a reference signal to produce an interferogram.

5. The method of claim 1, where the radiation source comprises a first optical fiber having a first exposed end.

6. The method of claim 1, wherein the radiation source is a light-emitting diode.

7. The method of claim 1, wherein the radiation source is a laser device.

8. The method of claim 1, wherein the radiation collector comprises a second optical fiber having a second exposed end.

9. The method of claim 1, wherein the radiation collector comprises an electrooptic sensor.

10. The method of claim 8, wherein the obtaining the sample signal comprises collecting radiation that has been transmitted through the tissue from the radiation source to the second exposed end, and

the determining the refractive index comprises comparing a physical distance from the radiation source to the second exposed end and a measured pathlength from the radiation source to the second exposed end.

11. The method of claim 8, wherein the radiation source comprises a first optical fiber having a first exposed end,

the obtaining the sample signal comprises collecting radiation that has been transmitted through the tissue from the first exposed end to the second exposed end, and

the determining the refractive index comprises comparing a physical distance from the first exposed end to the second exposed end and a measured pathlength from the first exposed end to the second exposed end.

12. The method of claim 1, wherein the obtaining the sample signal comprises collecting radiation that has been transmitted through the tissue from the radiation source to the radiation collector, and

the determining the refractive index comprises measuring the angle of displacement of radiation that has been transmitted through the tissue from the radiation source to the radiation collector.

13. The method of claim 12, wherein the radiation collector comprises an electrooptic sensor.

14. The method of claim 1, further comprising identifying the tissue by the refractive index.

15. A method of performing a biopsy of tissue, comprising:

identifying tissue according to the method of claim 14; and

biopsying at least a portion of the tissue.

16. The method of claim 1, wherein the distance between the radiation source and radiation collector is 50 micrometers to 2.0 millimeters.

17. A method of analyzing tissue, comprising:

inserting a probe into tissue;

impinging radiation upon the tissue;

obtaining a sample signal of the radiation from the tissue; and

determining a refractive index of the tissue from the sample signal;

wherein the probe comprises;

a housing,

a piercing tip, at a distal end of the housing,

a groove, in the housing,

a radiation source, and

a reflective surface,

wherein an optical path extends across the groove between the radiation source and the reflective surface.

18. The method of claim 17, further comprising determining at least one other optical property of the tissue.

19. The method of claim 18, wherein the at least one other optical property is selected from the group consisting of the attenuation coefficient, the scattering profile, the anisotropy factor, the birefringence, the spectral shift, and the texture.

20. The method of claim 17, wherein the radiation source comprises a first optical fiber having a first exposed end.

21. The method of claim 17, wherein the radiation source is a light-emitting diode.

22. The method of claim 17, wherein the radiation source is a laser device.

23. The method of claim 20, wherein the obtaining the sample signal comprises collecting radiation that has been reflected from the reflective surface to the first exposed end, and

the determining the refractive index comprises comparing a physical distance from the first exposed end to the reflective surface and a measured pathlength from the first exposed end to the reflective surface.

24. The method of claim 17, further comprising a second optical fiber having a second exposed end,

wherein the obtaining the sample signal comprises collecting radiation that has been reflected from the reflective surface to the second exposed end, and

the determining the refractive index comprises comparing a physical distance from the second exposed end to the reflective surface and a measured pathlength from the second exposed end to the reflective surface.

25. The method of claim 17, wherein the obtaining the sample signal comprises collecting radiation that has been reflected from the reflective surface, and

the determining the refractive index comprises measuring the angle of displacement of radiation that has been reflected from the reflective surface.

26. The method of claim 25, wherein radiation is collected by an electrooptic sensor.

27. The method of claim 17, further comprising identifying the tissue by the refractive index.

28. The method of claim 17, wherein the distance between the radiation source and reflective surface is 50 micrometers to 2.0 millimeters.