Tangential flow filtration devices and methods for stem cell enrichment
First Claim
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1. A method for separating stem cells from a sample from a subject wherein the sample comprises stem cells and non-stem cell constituents wherein the non-stem cells constituents can include cell population which have essentially the same size as the stem cells, the method comprising separation on a tangential flow filtration device by:
- (i) pretreating the sample to induce cell shrinkage of cell populations with essentially the same size as the stem cells;
(ii) introducing the sample into a remover unit (1) comprising a cross-flow chamber (3) through an inlet (6) in the remover unit;
(iii) subjecting the sample to cross-flow substantially parallel to a filter (5) having a pore size of about 1 to about 10 microns; and
(iv) selectively removing non-stem cell constituents from the sample to form a cell population enriched for stem cells.
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Abstract
The present invention provides methods for enriching a heterogenous mixture of bone marrow or blood constituents for stem cells by removal of non-stem cell constituents comprising separation of the non-stem cell constituents using a tangential flow filtration device.
26 Citations
27 Claims
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1. A method for separating stem cells from a sample from a subject wherein the sample comprises stem cells and non-stem cell constituents wherein the non-stem cells constituents can include cell population which have essentially the same size as the stem cells, the method comprising separation on a tangential flow filtration device by:
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(i) pretreating the sample to induce cell shrinkage of cell populations with essentially the same size as the stem cells; (ii) introducing the sample into a remover unit (1) comprising a cross-flow chamber (3) through an inlet (6) in the remover unit; (iii) subjecting the sample to cross-flow substantially parallel to a filter (5) having a pore size of about 1 to about 10 microns; and (iv) selectively removing non-stem cell constituents from the sample to form a cell population enriched for stem cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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26. A method for enriching a sample of bone marrow or blood constituents for stem cells, comprising:
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(i) pretreating the sample to induce cell shrinkage of cell populations with essentially the same size as the stem cells; (ii) introducing the sample into a tangential flow filtration (TFF) unit, the TFF unit comprising a cross-flow chamber, a filtrate chamber, and a filter in fluid communication with the cross-flow chamber and the filtrate chamber, the filter having a pore size of about 1 to about 10 microns; (iii) recirculating the sample through the TFF unit at a predetermined input rate and a predetermined filtration rate, the predetermined input rate at least five times the predetermined filtration rate;
wherein the predetermined filtration rate is less than the unopposed filtration rate for the filter; and(iv) isolating a cell population enriched for stem cells. - View Dependent Claims (27)
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Specification