Delta-9-tetrahydrocannabinol detection method
First Claim
1. A method for detecting in a liquid sample delta-9-tetrahydrocannabinol (THC) or one or more of THC and its metabolites 11-nor-9-carboxy-delta-9-THC (THC-COOH) and 11-hydroxy-delta-9-THC (THC-OH), which comprises:
- (a) contacting said sample with (i) a 5-pentylresorcinol/macromolecular carrier conjugate wherein 5-pentylresorcinol is conjugated via a hydroxyl group or a derivative thereof on its benzene ring to the carrier and (ii) an antibody capable of binding both THC and said conjugate; and
(b) determining whether binding of said antibody to said conjugate is reduced in the presence of said sample,wherein said antibody may exhibit cross-reactivity with said metabolites and reduction of the binding of said antibody to said conjugate is indicative of the presence of THC or the presence of one or more of THC, THC-COOH and THC-OH.
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Accused Products
Abstract
The invention provides competitive immunoassay techniques for high sensitivity detection of delta-9-tetrahydro-cannabinol (cannabis; THC) employing a carrier conjugate of an intermediate in the biosynthesis of cannabis, more particularly 5-pentylresorcinol conjugated to a macromolecular carrier via its hydroxyl groups. By employing such a conjugate with anti-THC antibody in a lateral flow immunochromatography test device convenient on-site testing for low levels of cannabis in liquid samples may be achieved. Such testing is particularly favoured for roadside testing for cannabis in oral fluid samples.
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Citations
28 Claims
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1. A method for detecting in a liquid sample delta-9-tetrahydrocannabinol (THC) or one or more of THC and its metabolites 11-nor-9-carboxy-delta-9-THC (THC-COOH) and 11-hydroxy-delta-9-THC (THC-OH), which comprises:
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(a) contacting said sample with (i) a 5-pentylresorcinol/macromolecular carrier conjugate wherein 5-pentylresorcinol is conjugated via a hydroxyl group or a derivative thereof on its benzene ring to the carrier and (ii) an antibody capable of binding both THC and said conjugate; and (b) determining whether binding of said antibody to said conjugate is reduced in the presence of said sample, wherein said antibody may exhibit cross-reactivity with said metabolites and reduction of the binding of said antibody to said conjugate is indicative of the presence of THC or the presence of one or more of THC, THC-COOH and THC-OH. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A test strip or sheet for use in a lateral flow analytical device for a carrying out a lateral flow immunochromatography assay, said test strip or sheet comprising:
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(i) a strip or sheet comprising a dry porous material having immobilised thereon in an analyte detection zone (a) a 5-pentylresorcinol/macromolecular carrier conjugate wherein 5-pentylresorcinol is conjugated via a hydroxyl group or a derivative thereof on its benzene ring to the carrier, or (b) an antibody capable of binding both THC and said conjugate; and (ii) joined to, or integral to, said strip or sheet providing said analyte detection zone, a label release zone containing either said antibody in labeled form, if said analyte detection zone presents immobilized 5-pentylresorcinol/carrier conjugate, or, if said analyte detection zone presents immobilized antibody, detectable 5-pentylresorcinol conjugate, whereby said labeled antibody or detectable conjugate will be released into a liquid sample drawn into the label release zone. - View Dependent Claims (17, 18, 19, 20, 21, 22)
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23. A lateral flow device, comprising a test strip or sheet, wherein said test strip or sheet comprises:
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(i) a strip or sheet comprising a dry porous material having immobilized thereon in an analyte detection zone (a) a 5-pentylresorcinol/macromolecular carrier conjugate wherein 5-pentylresorcinol is conjugated via a hydroxyl group or a derivative thereof on its benzene ring to the carrier, or (b) an antibody capable of binding both THC and said conjugate; and (ii) joined to, or integral to, said strip or sheet providing said analyte detection zone, a label release zone containing either said antibody in labeled form, if said analyte detection zone presents immobilized 5-pentylresorcinol/carrier conjugate, or, if said analyte detection zone presents immobilized antibody, detectable 5-pentylresorcinol conjugate, whereby said labeled antibody or detectable conjugate will be released into a liquid sample drawn into the label release zone.
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24. A kit comprising:
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(a) a 5-pentylresorcinol/macromolecular carrier conjugate wherein 5-pentylresorcinol is conjugated via a hydroxyl group or a derivative thereof on its benzene ring to the carrier; and (b) an antibody capable of binding both THC and said conjugate. - View Dependent Claims (25, 26, 27, 28)
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Specification