Polymerase chain reaction kit and method of manufacturing the same
First Claim
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1. A method of manufacturing a polymerase chain reaction kit comprising the steps of:
- a) forming a cavity for holding a sample solution, a barrier, and a flow channel for circulating a heating medium, in a surface of an integral, one-piece substrate, wherein the barrier separates the cavity and the flow channel so that the flow channel is adjacent to a boundary of the cavity defined by the barrier;
b) attaching the substrate to at least one of a plurality of cover plates, thereby sealing at least one of the cavity and the flow channel;
c) connecting the cavity to a sample-injection inlet exposed to outside; and
d) connecting the flow channel to a flow-in hole and a flow-out hole exposed to outside.
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Abstract
The present invention provides a kit employed for polymerase chain reaction. The kit has a cavity and a flow channel on a substrate. The flow channel is separated from the cavity by at least a barrier formed along the cavity. Such a structure allows the cavity to be filled with a sample solution even in minute quantities. At the same time, the structure can provide a sample solution with a rapid temperature-control. The structure can therefore contribute to accelerated polymerase chain reaction.
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12 Claims
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1. A method of manufacturing a polymerase chain reaction kit comprising the steps of:
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a) forming a cavity for holding a sample solution, a barrier, and a flow channel for circulating a heating medium, in a surface of an integral, one-piece substrate, wherein the barrier separates the cavity and the flow channel so that the flow channel is adjacent to a boundary of the cavity defined by the barrier; b) attaching the substrate to at least one of a plurality of cover plates, thereby sealing at least one of the cavity and the flow channel; c) connecting the cavity to a sample-injection inlet exposed to outside; and d) connecting the flow channel to a flow-in hole and a flow-out hole exposed to outside. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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Specification