Blood hemolysis analyzer
First Claim
1. A system for determining cell-free hemoglobin concentration within a sample containing at least one cell-free hemoglobin derivative and at least one cellular hemoglobin derivatives contained within erythrocytes, the system comprising:
- a light source configured to emit light;
a sample block containing the sample and configured to allow the light to pass through the sample wherein, a first portion of the light, having a wavelength of about 390-460 nm, is absorbed by the cell-free hemoglobin derivatives, and a second portion of the light, having a wavelength of about 390-460 nm, is absorbed by the cellular hemoglobin derivatives contained within erythrocytes;
at least one absorption detector configured to determine the light absorption of the sample block containing the sample, within a wavelength range of about 390-460 nm, and to generate a flattened spectra of cellular hemoglobin derivatives contained within erythrocytes and a non-flattened spectra of cell-free hemoglobin derivatives; and
a processor configured to use data from the absorption detector to compare the flattened spectra of cellular hemoglobin derivatives contained within erythrocytes to the non-flattened spectra of cell free hemoglobin derivatives determined from the change in the light absorption of the sample within a wavelength range of 390-460 nm, and thereby determine the cell free hemoglobin concentration within the sample.
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Accused Products
Abstract
Systems and methods for determining the concentration of hemoglobin derivatives in bodily fluids include devices for measuring and comparing the absorption of electromagnetic radiation by cellular and cell-free hemoglobin at two or more wavelengths in the Soret region. Systems and methods for determining erythrocyte membrane fragility include devices for measuring the concentration of at least one cell-free hemoglobin derivative, and using the absorption properties of cellular and cell-free hemoglobin derivatives in the Soret region.
50 Citations
22 Claims
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1. A system for determining cell-free hemoglobin concentration within a sample containing at least one cell-free hemoglobin derivative and at least one cellular hemoglobin derivatives contained within erythrocytes, the system comprising:
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a light source configured to emit light; a sample block containing the sample and configured to allow the light to pass through the sample wherein, a first portion of the light, having a wavelength of about 390-460 nm, is absorbed by the cell-free hemoglobin derivatives, and a second portion of the light, having a wavelength of about 390-460 nm, is absorbed by the cellular hemoglobin derivatives contained within erythrocytes; at least one absorption detector configured to determine the light absorption of the sample block containing the sample, within a wavelength range of about 390-460 nm, and to generate a flattened spectra of cellular hemoglobin derivatives contained within erythrocytes and a non-flattened spectra of cell-free hemoglobin derivatives; and a processor configured to use data from the absorption detector to compare the flattened spectra of cellular hemoglobin derivatives contained within erythrocytes to the non-flattened spectra of cell free hemoglobin derivatives determined from the change in the light absorption of the sample within a wavelength range of 390-460 nm, and thereby determine the cell free hemoglobin concentration within the sample. - View Dependent Claims (2, 3, 4)
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5. A method for determining cell-free hemoglobin concentration within a sample containing at least one cell-free hemoglobin derivative and at least one cellular hemoglobin derivative contained within erythrocytes, the method comprising:
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projecting light with a light source configured to emit light into a sample block containing the sample and configured to allow the light to pass through the sample wherein, a first portion of the light, having a wavelength of about 390-460 nm, is absorbed by the cell-free hemoglobin derivative, and a second portion of the light, having a wavelength of about 390-460 nm, is absorbed by the cellular hemoglobin derivative contained within erythrocytes; measuring the light absorption with at least one absorption detector configured to determine the light absorption of the sample block containing the sample, within a wavelength range of about 390-460 nm, and to generate a flattened spectra of cellular hemoglobin derivatives contained within erythrocytes and a non-flattened spectra of cell-free hemoglobin derivatives; and utilizing a processor to compare the flattened spectra of cellular hemoglobin derivatives contained within erythrocytes to the non-flattened spectra of cell free hemoglobin derivatives determined from the measured change in the light absorption of the sample within a wavelength range of 390-460 nm, and thereby determine the cell free hemoglobin concentration within the sample. - View Dependent Claims (6, 7, 8, 9, 10, 11)
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12. A system for determining erythrocyte membrane fragility in a sample containing at least one cell-free hemoglobin derivative and at least one cellular hemoglobin derivative contained within erythrocytes, the system comprising:
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a light source configured to emit light; an erythrocyte stressor configured to apply a defined level of stress to the sample; a sample block containing the sample, after it has received a defined level of stress from the erythrocyte stressor, and configured to allow the light to pass through the sample wherein, a first portion of the light, having a wavelength of about 390-460 nm, is absorbed by the cell-free hemoglobin derivatives, and a second portion of the light, having a wavelength of about 390-460 nm, is absorbed by the cellular hemoglobin derivatives contained within erythrocytes; at least one absorption detector configured to determine the light absorption of the sample block containing the sample, within a wavelength range of about 390-460 nm, and to generate a flattened spectra of cellular hemoglobin derivatives contained within erythrocytes and a non-flattened spectra of cell-free hemoglobin derivatives; and a processor configured to use data from the absorption detector to compare the flattened spectra of cellular hemoglobin derivatives contained within erythrocytes to the non-flattened spectra of cell free hemoglobin derivatives determined from the change in the light absorption of the sample within a wavelength range of 390-460 nm, and thereby determine the cell free hemoglobin concentration within the sample, said concentration then being used to calculate the fraction of cells which have become lysed under the defined level of stress, with said fraction representing erythrocyte membrane fragility. - View Dependent Claims (13, 14, 15)
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16. A method for determining erythrocyte membrane fragility in a sample containing at least one cell-free hemoglobin derivative and at least one cellular hemoglobin derivative contained within erythrocytes, the method comprising:
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stressing the sample with an erythrocyte stressor configured to apply a defined level of stress to the sample; projecting light with a light source configured to emit light into a sample block containing the sample, which has been stressed, and configured to allow the light to pass through the sample wherein, a first portion of the light, having a wavelength of about 390-460 nm, is absorbed by the cell-free hemoglobin derivatives, and a second portion of the light, having a wavelength of about 390-460 nm, is absorbed by the cellular hemoglobin derivatives contained within erythrocytes; measuring the light absorption with at least one absorption detector configured to determine the light absorption of the sample block containing the sample, within a wavelength range of about 390-460 nm, and to generate a flattened spectra of cellular hemoglobin derivatives contained within erythrocytes and a non-flattened spectra of cell-free hemoglobin derivatives; and utilizing a processor to compare the flattened spectra of cellular hemoglobin derivatives contained within erythrocytes to the non-flattened spectra of cell free hemoglobin derivatives determined from the measured change in the light absorption of the sample within a wavelength range of 390-460 nm, and thereby determine the cell free hemoglobin concentration within the sample, said concentration then being used to calculate the fraction of cells which have become lysed under the defined level of stress, with said fraction representing erythrocyte membrane fragility. - View Dependent Claims (17, 18, 19, 20, 21, 22)
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Specification