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Metering technique for lateral flow assay devices

  • US 7,790,471 B2
  • Filed: 10/05/2007
  • Issued: 09/07/2010
  • Est. Priority Date: 12/13/2005
  • Status: Expired due to Fees
First Claim
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1. A method for performing a lateral flow assay, the method comprising:

  • providing a sampling membrane with a receiving region, a collection region and a detection region;

    defining a barrier between the collection region and the detection region so that the collection region has a known saturation volume that is less than about 100 microliters;

    supplying a test sample to a receiving region of the membrane and saturating the collection region with the test sample so that a known volume of the test sample is contained in the collection region that corresponds to the known saturation volume of the collection region;

    removing the barrier between the collection region and detection region of the membrane;

    supplying a diluent to the collection region of the membrane to facilitate flow of the test sample from the collection region to the detection region of the membrane; and

    defining the collection region by separating the collection region from the receiving region prior to supplying the diluent to the collection region.

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