Massive parallel method for decoding DNA and RNA
DC CAFCFirst Claim
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1. A nucleotide having a base that is attached to a detectable label through a cleavable linker, wherein the nucleotide has a deoxyribose comprising a cleavable chemical group capping the 3′
- OH group of the deoxyribose and wherein both the cleavable linker and the capping cleavable chemical group are cleavable by light.
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Abstract
This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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33 Claims
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1. A nucleotide having a base that is attached to a detectable label through a cleavable linker, wherein the nucleotide has a deoxyribose comprising a cleavable chemical group capping the 3′
- OH group of the deoxyribose and wherein both the cleavable linker and the capping cleavable chemical group are cleavable by light.
- View Dependent Claims (2, 3, 4, 5)
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6. A nucleotide having a base that is linked to a detectable label via a cleavable linker, wherein the nucleotide has a deoxyribose sugar moiety comprising a protecting or capping group attached via the 3′
- oxygen and the cleavable linker and the protecting or capping group are cleavable by light.
- View Dependent Claims (7, 8, 9, 10, 11)
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12. A nucleotide having a base that is attached to a detectable label through a cleavable linker, wherein the nucleotide has a deoxyribose comprising a cleavable chemical group capping the 3′
- OH group, wherein the cleavable linker is cleaved by a means selected from the group consisting of one or more of a physical means, a chemical means, a physical chemical means, heat, and light, and wherein the cleavable chemical group capping the 3′
OH group is cleaved by a means selected from the group consisting of one or more of a physical means, a chemical means, a physical chemical means, heat, and light. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
- OH group, wherein the cleavable linker is cleaved by a means selected from the group consisting of one or more of a physical means, a chemical means, a physical chemical means, heat, and light, and wherein the cleavable chemical group capping the 3′
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