Tissue collection device and methods

• US 7,794,408 B2
• Filed: 03/28/2003
• Issued: 09/14/2010
• Est. Priority Date: 03/28/2003
• Status: Active Grant

• Current Assignee: DePuy Mitek Holding Corporation (Johnson & Johnson), Depuy Synthes Products Incorporated (Johnson & Johnson), DePuy Synthes Products LLC (Johnson & Johnson)
• Original Assignee: Ethicon Incorporated (Johnson & Johnson)
• Inventors: Lepke, Steve, Binette, Francois, Hwang, Julia, Hyman, Eric, Perkins, Ashley, Orr, Keith M.
• Primary Examiner(s): Hindenburg; Max
• Assistant Examiner(s): TOWA, RENE T
• Application Number:
  US10/402,266
• Publication Number:
  US 20040193071A1
• Time in Patent Office: 2,727 Days
• Field of Search: 600/562, 600/563, 600/572, 600/573, 600/576, 600/577, 600/578, 600/579, 604/187, 604/198, 604/207, 604/218, 604/237, 604/119
• US Class Current: 600/562
• CPC Class Codes: A61B 10/0096 Casings for storing test samples A61B10/0038 and A61B10/0045 take precedence; preservation of living parts of the human or animal body A01N1/02; containers for retaining a material to be analysed B01L3/50; containers for enzymology or microbiology C12M1/16; swab-sampler being part of enzymology or microbiology container C12M1/30
• CPC Class Codes: A61F 2/4644 Preparation of bone graft, bone plugs or bone dowels, e.g. grinding or milling bone material bone material A61F2/28; disintegrating or milling devices B02C
• CPC Class Codes: A61F 2002/2835 Bone graft implants for filling a bony defect or an endoprosthesis cavity, e.g. by synthetic material or biological material
• CPC Class Codes: A61L 27/36 containing ingredients of undetermined constitution or reaction products thereof , e.g. transplant tissue, natural bone, extracellular matrix isolated natural compounds, e.g. collagen A61L27/24

Abstract

A biological tissue collection device is provided having a housing with a fluid-retaining inner chamber adapted to retain a bioimplantable, fluid permeable tissue scaffold. The scaffold is preferably retained in the housing in such a way that the scaffold separates the fluid-retaining inner chamber into a first chamber and a second chamber. The collection device further includes a driver mechanism coupled to the housing and effective to create a force within the housing to displace fluid disposed within the second chamber to the first chamber. As the fluid is displaced, any biological tissue deposited on the tissue scaffold is dispersed within the fluid. Removal of the force enables the fluid to return to the second chamber and thereby deposit the tissue onto the tissue scaffold. In an exemplary embodiment, the tissue is deposited evenly onto the tissue scaffold.

Claims

1. A biological tissue collection device, comprising:

a housing having a fluid-retaining inner chamber with a bioimplantable, fluid permeable tissue scaffold disposed therein such that the scaffold separates the fluid-retaining inner chamber into a first chamber and a second chamber, wherein the second chamber includes an overflow mechanism located just distal to the scaffold and effective to allow any excess fluid disposed within the second chamber to be released;

a driver mechanism coupled to the housing and effective to create a force within the housing to displace fluid disposed within the second chamber to the first chamber and thereby disperse any biological tissue deposited on the tissue scaffold within the fluid, wherein removal of the force enables the fluid to return to the second chamber and thereby deposit the biological tissue onto the tissue scaffold; and

a jet plate disposed within the second chamber and movable between an original position, in which the jet plate is positioned a distance apart from the first chamber, and a second position, in which the driver mechanism applies a force that causes the jet plate to move toward the first chamber, the jet plate being adapted to agitate fluid disposed within the second chamber as the jet plate moves between the original position and the second position such that biological tissue is uniformly deposited onto the tissue scaffold.

2. The biological tissue collection device of claim 1, wherein the jet plate, when disposed in the second position, is effective to apply pressure to a surface of the tissue scaffold to force any air bubbles formed on the tissue scaffold to flow into the first chamber.

3. The biological tissue collection device of claim 1, wherein the jet plate comprises a body having at least one channel extending therethrough for allowing fluid to flow through the jet plate.

4. The biological tissue collection device of claim 3, wherein the jet plate includes upper and lower surfaces, and wherein the at least one channel is oriented at an angle and extends between the upper and lower surfaces.

5. The biological tissue collection device of claim 4, wherein the at least one channel extends from a perimeter of the lower surface of the jet plate to a center of the upper surface of the jet plate.

6. The biological tissue collection device of claim 1, wherein the first chamber is disposed upstream of the tissue scaffold, and the second chamber is disposed downstream of the tissue scaffold.

7. The biological tissue collection device of claim 1, wherein the first chamber includes at least one entry port for delivering fluid into the first chamber.

8. The biological tissue collection device of claim 7, wherein the at least one entry port includes a flow control valve effective to control the flow of fluid therethrough.

9. The biological tissue collection device of claim 7, wherein the driver mechanism comprises a plunger coupled to the first chamber, and wherein the at least one entry port is formed through the plunger.

10. The biological tissue collection device of claim 1, wherein the driver mechanism is coupled to the first chamber and is effective to create a vacuum force to pull fluid from the second chamber into the first chamber.

11. The biological tissue collection device of claim 10, wherein the driver mechanism is selected from the group consisting of at least one plunger, a charged gas cylinder, and a vacuum pump.

12. The biological tissue collection device of claim 1, wherein the driver mechanism is coupled to the second chamber and is effective to create a force to push fluid from the second chamber into the first chamber.

13. The biological tissue collection device of claim 12, wherein the driver mechanism is selected from the group consisting of at least one plunger, a charged gas cylinder, and a vacuum pump.

14. The biological tissue collection device of claim 10, wherein the driver mechanism is formed integrally with the first chamber such that the first chamber is movable between a first position and a second position to create a vacuum force to draw fluid from the second chamber into the first chamber.

15. The biological tissue collection device of claim 1, wherein the overflow mechanism is selected from the group consisting of a channel, a port, and a valve.

16. The biological tissue collection device of claim 1, further comprising a retaining member effective to retain a tissue scaffold within the chamber.

17. The biological tissue collection device of claim 16, wherein the retaining member comprises a wire mesh disposed within the housing between the first and second chambers, the wire mesh being adapted to seat a tissue scaffold.

18. The biological tissue collection device of claim 16, wherein the retaining member comprises a clamping ring effective to clamp an outer perimeter of a tissue scaffold.

19. The biological tissue collection device of claim 16, wherein the retaining member is removably disposed within the inner chamber of the housing.

20. The biological tissue collection device of claim 19, wherein the housing includes a transverse slot formed therein for removably receiving the retaining member.

21. The biological tissue collection device of claim 19, wherein the housing comprises a first portion defining the first inner chamber, and a second portion defining the second inner chamber, the first and second portions being adapted to removably mate to one another and to receive a tissue scaffold therebetween.

22. The biological tissue collection device of claim 21, wherein the first and second portions define the retaining member.

23. The biological tissue collection device of claim 1, wherein the housing includes a proximal end, a distal end, and a sidewall extending therebetween and defining the fluid-retaining inner chamber, and wherein the first chamber is formed within a proximal portion of the housing, and the second chamber is formed within a distal portion of the housing.

24. The biological tissue collection device of claim 23, wherein the driver mechanism comprises a plunger disposed within the first chamber and movable between a first position, in which the plunger is positioned at a distal end of the first chamber, and a second position, in which the plunger is positioned adjacent the proximal end of the housing.

25. The biological tissue collection device of claim 24, wherein movement of the plunger from the first position to the second position is effective to create a vacuum within the fluid-retaining inner chamber of the housing to draw fluid disposed within the second chamber into the first chamber.

26. The biological tissue collection device of claim 25, further comprising a driver release mechanism coupled to the first chamber and effective to release the vacuum within the fluid-retaining inner chamber of the housing.

27. The biological tissue collection device of claim 26, wherein the driver release mechanism comprises an air flow entry port formed in the sidewall of the housing adjacent the proximal end of the housing and effective to allow air to enter the fluid-retaining inner chamber when the plunger is positioned proximal to the air flow entry port.

28. A biological tissue collection device, comprising:

a housing having a proximal chamber with at least one entry port for receiving fluid, a distal chamber, and a tissue scaffold retaining member disposed therebetween, wherein the distal chamber includes an overflow mechanism located just distal to the scaffold and effective to allow any excess fluid disposed within the distal chamber to be released;

a driver mechanism coupled to the proximal chamber and effective to create a fluid driving force within the proximal and distal chambers;

a driver release mechanism formed in one of the proximal distal chambers and that is effective to release the force applied by the driver mechanism; and

a jet plate movably disposed within the distal chamber that, when the driver mechanism is activated, is effective to agitate fluid disposed within the distal chamber to uniformly deposit biological tissue onto a tissue scaffold retained within the tissue scaffold retaining member.

29. The biological tissue collection device of claim 28, wherein the retaining member is removably disposed within the housing.

30. A method of preparing a tissue scaffold, comprising the steps of:

providing a biological tissue collection device having

a housing having a fluid-retaining inner chamber;

a bioimplantable, fluid permeable tissue scaffold disposed within the fluid-retaining inner chamber of the housing such that the tissue scaffold separates the fluid-retaining inner chamber into a first chamber and a second chamber, the second chamber including an overflow mechanism located just distal to the scaffold and effective to allow any excess fluid disposed within the second chamber to be released,

a driver mechanism coupled to the housing and effective to create a force within the housing, and

a jet plate disposed within the second chamber;

delivering a biological tissue-containing fluid into the first chamber, wherein the biological tissue is deposited onto the tissue scaffold and the fluid flows though the tissue scaffold and into the second chamber;

activating the driver mechanism to cause fluid to be displaced from the second chamber and into the first chamber, the driver mechanism causing the jet plate to agitate the fluid such that any biological tissue deposited on the tissue scaffold is dispersed within the fluid; and

releasing the driver mechanism to enable the fluid to return to the second chamber and thereby uniformly deposit the biological tissue onto the tissue scaffold.

31. The method of claim 30, wherein the driver mechanism is coupled to the first chamber and is effective to create a vacuum force to draw fluid from the second chamber into the first chamber.

32. The method of claim 30, wherein the driver mechanism is coupled to the second chamber and is effective to create a force to push fluid from the second chamber into the first chamber.

33. The method of claim 30, wherein the jet plate disposed within the second chamber moves between an original position, in which the jet plate is positioned a distance apart from the tissue scaffold, and a second position, in which the driver mechanism applies a force that causes the jet plate to contact the tissue scaffold.

34. The method of claim 33, wherein the jet plate applies a pressure to a surface of the tissue scaffold in the second position to compress any air bubbles formed on the tissue scaffold.

35. The method of claim 33, wherein the jet plate comprises a body having at least one channel extending therethrough, the fluid flowing though the channel in the jet plate.

36. The method of claim 30, further comprising releasing any excess fluid disposed within the second chamber through the overflow mechanism.