Amperometric sensor for uric acid and method for the same
First Claim
1. A manufacturing method for an amperometric sensor for uric acid, comprising the steps of:
- (a) fixing a conducting wire on a carbon electrode with a silver paste and then baking the carbon electrode in an oven, said conducting wire comprising aluminum;
(b) packaging the portion of the sensor device containing the conducting wire with epoxy and then baking the sensor in an oven to manufacture a tri-polar carbon electrode;
(c) forming the tri-polar carbon electrode having a sensing window thereon;
(d) mixing polyacrylamide and a phosphate solution;
(e) forming a polyacrylamide solution by mixing the polyacrylamide used in step (d) with ferrocenecarboxylic acid;
(f) diluting uricase with the polyacrylamide solution obtained in step (e); and
(g) dripping a proper amount of the diluted uricase obtained in step (f) on the sensing window, and distributing the diluted uricase uniformly thereon, and then air-drying the diluted uricase to fix it, and the amperometric sensor for uric acid is achieved.
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Abstract
An amperometric sensor for uric acid and a manufacturing method thereof are disclosed, in which polyacrylamide is used to fix catalase, uricase and ferrocenecarboxylic acid on a working electrode. In determining concentration of uric acid, hydrogen peroxide is produced when enzyme and uric acid react with each other and then a reduction current generated from enzyme on the electrode with an external voltage 200 mV applied is detected. In determining concentration of uric acid, a concentration range of 2.5-20 mg/dl is achieved and sensibility of the sensor in a linear portion is 5.17 uAcm−2(mg/dl)−1. In addition, reaction time required for the reaction between enzyme and uric acid is 5.17 uAcm−2(mg/dl)−1.
3 Citations
8 Claims
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1. A manufacturing method for an amperometric sensor for uric acid, comprising the steps of:
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(a) fixing a conducting wire on a carbon electrode with a silver paste and then baking the carbon electrode in an oven, said conducting wire comprising aluminum; (b) packaging the portion of the sensor device containing the conducting wire with epoxy and then baking the sensor in an oven to manufacture a tri-polar carbon electrode; (c) forming the tri-polar carbon electrode having a sensing window thereon; (d) mixing polyacrylamide and a phosphate solution; (e) forming a polyacrylamide solution by mixing the polyacrylamide used in step (d) with ferrocenecarboxylic acid; (f) diluting uricase with the polyacrylamide solution obtained in step (e); and (g) dripping a proper amount of the diluted uricase obtained in step (f) on the sensing window, and distributing the diluted uricase uniformly thereon, and then air-drying the diluted uricase to fix it, and the amperometric sensor for uric acid is achieved. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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Specification
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- Resources
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Current AssigneeChung-Yuan Christian University
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Original AssigneeChung-Yuan Christian University
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InventorsHsiung, Shen-Kan, Cheng, Mei-Ling, Sun, Tai-Ping, Chou, Jung-Chuan
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Primary Examiner(s)Nguyen; Nam X
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Assistant Examiner(s)CARLSON, KOURTNEY SALZMAN
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Application NumberUS11/211,679Publication NumberTime in Patent Office1,845 DaysField of Search20440301-40315, 435/174, 435/288, 435/817, 427/2.13, 427/58, 438/48US Class Current427/2.13CPC Class CodesG01N 27/26 by investigating electroche...
