Detection and measurement of thyroid analyte profile
First Claim
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1. A non radioisotopic method of determining canine T4AA in a patient sample comprising:
- (a) measuring the amount of Free T4 in a patient sample, wherein the sample is from a canine species, the Free T4 being attached as Free T4/T4AA complex when T4AA is present in the patient sample together with Free T4 unattached to T4AA;
(b) treating the patient sample with a non-radioisotopic precipitating or binding agent to precipitate or bind Free T4/T4AA complex thereby forming a complex of precipitated or bound Free T4/T4AA complex;
(c) suspending the non-radioisotopic precipitate or bound complex, and at least one of;
(i) measuring the amount of Free T4/T4AA complex in suspended non-radioisotopic precipitate or bound complex using a non-radioisotopic label, and(ii) subtracting the measurement of i) from a) wherein the result of ii) is directly proportional to the amount of Free T4/T4AA complex thereby determining the amount of T4AA in the patient sample;
or(iii) measuring Free T4 in supernatant or surrounding fluid of the non-radioisotopic precipitate or bound complex using a non-radioisotopic label;
(iv) subtracting the measurement of iii) from a) wherein the result of iv) is indirectly proportional to the amount of Free T4/T4AA complex thereby determining the amount of T4AA in the patient sample;
effecting detection with a sensitivity in a range between 1 picogram per mL and up to about 1 femtogram per mL.
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Abstract
A non-radioisotopic method of detecting thyroid analytes comprising detecting T3, Free T3, T4, Free T4 and thyroglobulin autoantibody in a sample of a non-human species. Each one of these analytes in an assay profile includes non-radio isotopic measurement of T3, Free T3, T4, Free T4 and thyroglobulin autoantibody in the sample from the non-human species. A non-radioisotopic method detects T3AA and T4AA thyroid autoantibodies in a sample from a non-human species such as the canine species. A non-radioisotopic method detects Free T4 in a sample of a non-human species.
25 Citations
13 Claims
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1. A non radioisotopic method of determining canine T4AA in a patient sample comprising:
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(a) measuring the amount of Free T4 in a patient sample, wherein the sample is from a canine species, the Free T4 being attached as Free T4/T4AA complex when T4AA is present in the patient sample together with Free T4 unattached to T4AA; (b) treating the patient sample with a non-radioisotopic precipitating or binding agent to precipitate or bind Free T4/T4AA complex thereby forming a complex of precipitated or bound Free T4/T4AA complex; (c) suspending the non-radioisotopic precipitate or bound complex, and at least one of; (i) measuring the amount of Free T4/T4AA complex in suspended non-radioisotopic precipitate or bound complex using a non-radioisotopic label, and (ii) subtracting the measurement of i) from a) wherein the result of ii) is directly proportional to the amount of Free T4/T4AA complex thereby determining the amount of T4AA in the patient sample; or (iii) measuring Free T4 in supernatant or surrounding fluid of the non-radioisotopic precipitate or bound complex using a non-radioisotopic label; (iv) subtracting the measurement of iii) from a) wherein the result of iv) is indirectly proportional to the amount of Free T4/T4AA complex thereby determining the amount of T4AA in the patient sample; effecting detection with a sensitivity in a range between 1 picogram per mL and up to about 1 femtogram per mL. - View Dependent Claims (9)
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2. A non radioisotopic method of determining canine T4AA in a sample:
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(a) measuring the amount of Free T4 in a patient sample, wherein the sample is from a canine species, the Free T4 being attached as Free T4/T4AA complex when T4AA is present in the patient sample together with Free T4 unattached to T4AA; (b) treating the patient sample with a non-radioisotopic precipitating or binding agent to precipitate or bind Free T4/T4AA complex thereby forming a complex of precipitated or bound FreeT4 and T4AA; (c) suspending the non-radioisotopic precipitate or bound complex, and at least one of; (i) measuring the amount of Free T4/T4AA complex in suspended non-radioisotopic precipitate or bound complex using a non-radioisotopic label; and (ii) subtracting the measurement of i) from a) wherein the result of ii) is directly proportional to the amount of Free T4/T4AA complex thereby determining the amount of T4AA in the patient sample; or (iii) measuring Free T4 in supernatant or surrounding fluid of the precipitate or bound complex using a non-radioisotopic label; and (iv) subtracting the measurement of iii) from a) wherein the result of iv) is indirectly proportional to the amount of FreeT4/T4AA complex thereby determining the amount of T4AA in the patient sample; and effecting detection of a), c) i) or c) iii) by employing non-radioisotopic chemiluminesence, and including a bioluminescent detector and microparticles for immobilizing Free T4 as a solid phase, thereby to obtain a non-radioisotopic determination of T4AA. - View Dependent Claims (7, 10, 12)
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3. A non radioisotopic method of determining canine T4AA in a sample:
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(a) measuring the amount of Free T4 in a patient sample, wherein the sample is from a canine species, the Free T4 being attached as Free T4/T4AA complex when T4AA is present in the patient sample together with Free T4 unattached to T4AA; (b) treating the patient sample with a non-radioisotopic precipitating or binding agent to precipitate or bind FreeT4/T4AA complex thereby forming a complex of precipitated or bound FreeT4 and T4AA; (c) suspending the non-radioisotopic precipitate or bound complex, and at least one of; (i) measuring the amount of Free T4/T4AA complex in suspended non-radioisotopic precipitate or bound complex using a non-radioisotopic label, and (ii) subtracting the measurement of i) from a) wherein the result of ii) is directly proportional to the amount of FreeT4/T4AA complex thereby determining the amount of T4AA in the patient sample; or (iii) measuring Free T4 in supernatant or surrounding fluid of the precipitate or bound complex using a non-radioisotopic label; and (iv) subtracting the measurement of iii) from a) wherein the result of iv) is indirectly proportional to the amount of FreeT4/T4AA complex thereby determining the amount of T4AA in the patient sample; and (d) effecting detection of a), c) i) or c) iii) by employing a non-radioisotopic detection thereby to obtain a non-radioisotopic determination of T4AA. - View Dependent Claims (4, 5, 6, 8, 11, 13)
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Specification