Extracellular matrix composite materials, and manufacture and use thereof

• US 7,795,027 B2
• Filed: 03/03/2006
• Issued: 09/14/2010
• Est. Priority Date: 09/04/2003
• Status: Active Grant

• Current Assignee: Cook Biotech Incorporated (Cook Group Incorporated)
• Original Assignee: Cook Biotech Incorporated (Cook Group Incorporated)
• Inventors: Hiles, Michael C
• Primary Examiner(s): LI, QIAN JANICE
• Application Number:
  US11/368,077
• Publication Number:
  US 20060147433A1
• Time in Patent Office: 1,656 Days
• Field of Search: None
• US Class Current: 435/395
• CPC Class Codes: A61K 35/22 Urine; Urinary tract, e.g. kidney or bladder; Intraglomerular mesangial cells; Renal mesenchymal cells; Adrenal gland
• CPC Class Codes: A61K 35/37 Digestive system
• CPC Class Codes: A61K 35/44 Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells
• CPC Class Codes: A61L 27/3629 Intestinal tissue, e.g. small intestinal submucosa
• CPC Class Codes: A61L 27/3633 Extracellular matrix [ECM]
• CPC Class Codes: A61L 27/3641 characterised by the site of application in the body materials for artificial blood vessels A61L27/507; materials for use in artificial skin A61L27/60
• CPC Class Codes: A61L 27/3645 Connective tissue
• CPC Class Codes: A61L 27/3804 characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
• CPC Class Codes: A61L 27/48 with macromolecular fillers
• CPC Class Codes: C08L 89/00 Compositions of proteins; Compositions of derivatives thereof foodstuff preparations A23J3/00

Abstract

Described are preferred extracellular matrix composites including a first extracellular matrix material having a second extracellular matrix material deposited thereon. The preferred materials are made by culturing cells in contact with an extracellular matrix graft material in a fashion to cause the cells to biosynthesize and deposit extracellular matrix components on the material. The cells are then removed to provide the extracellular matrix composite material.

Claims

1. A method for preparing an acellular extracellular matrix composite, comprising:

providing a first extracellular matrix material having a surface;

culturing cells in vitro under conditions to secrete a second extracellular matrix material wherein said second material is deposited upon said surface by said cells to form an extracellular matrix composite comprising said first extracellular matrix material with said second extracellular matrix material on said surface;

removing said cells; and

isolating the extracellular matrix composite in acellular form.

2. The method of claim 1, wherein said second extracellular matrix material comprises one or more fiber-forming proteins.

3. The method of claim 2, wherein the one or more fiber-forming proteins include collagen and/or elastin.

4. The method of claim 1, wherein said second extracellular matrix material comprises one or more adhesive proteins.

5. The method of claim 4, wherein said adhesive proteins comprise fibronectin and/or laminin.

6. The method of claim 1, wherein the first extracellular matrix material comprises submucosa.

7. The method of claim 6, wherein the submucosa is intestinal, urinary bladder or stomach submucosa.

8. The method of claim 7, wherein the submucosa is small intestinal submucosa.

9. The method of claim 1, wherein the first extracellular matrix material is from porcine.

10. The method of claim 1, wherein the second extracellular matrix material is from human.

11. The method of claim 1, wherein the second extracellular matrix material is secreted by one or more of endothelial cells, muscle cells, fibroblast cells, mesothelial cells, pericyte cells, macrophage cells, monocyte cells, plasma cells, mast cells, adipocyte cells, chondrocyte cells, stem cells, or a cell population differentiated from stem cells.

12. The method of claim 11, wherein the second extracellular matrix material contains matrix components secreted by endothelial cells.

13. The method of claim 12, wherein the endothelial cells are vascular endothelial cells.

14. The method of claim 1, wherein said isolating comprises rendering the extracellular matrix composite substantially devoid of cells and cell components.

15. A method of making a conditioned extracellular matrix graft prosthesis, comprising:

providing a first extracellular matrix material;

culturing cells in contact with said extracellular matrix material;

causing said cells to synthesize and deposit a second extracellular matrix material upon said first extracellular matrix material; and

removing said cells to form an extracellular matrix graft prosthesis containing said first and second extracellular matrix materials and essentially free of said cells and membranes thereof.

16. The method of claim 15, wherein said providing comprises processing the first extracellular matrix material to be free of cells from a source tissue.

17. The method of claim 16, wherein the first extracellular matrix material is isolated in an intact sheet form.

18. The method of claim 15, wherein said culturing cells comprises culturing endothelial cells, muscle cells, fibroblast cells, mesothelial cells, pericyte cells, macrophage cells, monocyte cells, plasma cells, mast cells, adipocyte cells, chondrocyte cells, stem cells, or a cell population differentiated from stem cells.

19. The method of claim 15, wherein said culturing cells comprises culturing epithelial cells.

20. The method of claim 19, wherein the epithelial cells are corneal epithelial cells or glomerular epithelial cells.

21. The method of claim 15, wherein, during said culturing, the cells and the extracellular matrix are subjected to stress.

22. The method of claim 21, wherein the stress is mechanical, chemical or physical stress.

23. The method of claim 15, wherein the second extracellular matrix material comprises one or more fiber-forming proteins.

24. The method of claim 23, wherein the one or more fiber forming proteins include collagen and/or elastin.

25. The method of claim 15, wherein the second extracellular matrix material comprises one or more adhesive proteins.

26. The method of claim 25, wherein the adhesive proteins comprise fibronectin and/or laminin.

27. The method of claim 15, wherein the second extracellular matrix material comprises one or more of type I and type III collagens, fibronectin, tenascin, glycosaminoglycans, acidic and basic fibroblast growth factor, transforming growth factor-beta, keratinocyte growth factor, and decorin.